THE USE OF COMPUTER-ASSISTED VIDEO IMAGE-ANALYSIS IN THE ENUMERATION OF IMMUNOSTAINED CELLS IN TISSUE-SECTIONS
THE USE OF COMPUTER-ASSISTED VIDEO IMAGE-ANALYSIS IN THE ENUMERATION OF IMMUNOSTAINED CELLS IN TISSUE-SECTIONS
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DOI：
10.1016/0022-1759(90)90193-y
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发表时间：
1990-08-07
影响因子：
2.2
通讯作者：
BRADLEY, J
中科院分区：
文献类型：
--
作者：
NORAZMI, MN;HOHMANN, AW;BRADLEY, J
一种新的计算机辅助视频图像分析(VIA)方法被用来确定组织切片中免疫染色细胞的数量。这种方法可以对特定表型的细胞进行准确和客观的量化。这种计数是通过测量被测试的单抗(如T细胞标记物CD3)染色的面积并将其与白细胞共同(LCA)单抗(CD45)染色的面积进行比较来实现的。然后计算T细胞在特定组织中的总白细胞群中的比例。通过设置计算机检测染色强度的阈值，一致地维持阳性(染色)和阴性(未染色)细胞的分化。这使得能够在组织切片内以及在用相同抗体染色的切片之间保持一致性。在本研究中，我们用VIA测定了结肠癌中白细胞的表型，并将其与正常目测分析结果进行了比较。与常规目视分析相比，VIA法显示出明显的优势，尤其是在含有中等数量染色细胞的切片上。
A novel method of computer-assisted video image analysis (VIA) was used to determine the number of immunostained cells in tissue sections. This method permitted an accurate and objective quantification of cells of a particular phenotype. This enumeration was achieved by measuring the area stained by a test monoclonal antibody (such as the T cell marker, CD3) and comparing it with the area stained by a leukocyte common (LCA) monoclonal antibody (CD45). The proportion of T cells within the total leukocyte population in a particular tissue was then calculated. The differentiation of positive (stained) and negative (unstained) cells was uniformly maintained by setting the computer to detect a threshold for staining intensity. This enabled consistency to be maintained within a tissue section as well as between sections stained with the same antibody. In the present study, we determined the phenotype of leukocytes in colonic carcinomas by VIA and compared this with results obtained by normal visual analysis. The VIA method showed distinct advantages over normal visual analysis especially in section which contained moderate numbers of stained cells.