DIFFERENTIATION OF INNER-EAR FIBROCYTES ACCORDING TO THEIR ION-TRANSPORT RELATED ACTIVITY
DIFFERENTIATION OF INNER-EAR FIBROCYTES ACCORDING TO THEIR ION-TRANSPORT RELATED ACTIVITY
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DOI：
10.1016/0378-5955(91)90153-z
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发表时间：
1991-11-01
期刊：
影响因子：
2.8
通讯作者：
SCHULTE, BA
中科院分区：
文献类型：
--
作者：
SPICER, SS;SCHULTE, BA
通过Na，K-ATPase、碳酸氢酶(CA)和肌酸激酶(CK)等转运介导酶的免疫组织化学染色，证实了沙土鼠耳蜗侧壁和边缘的纤维细胞具有离子转运活性。螺旋韧带的纤维细胞不同于皱纹上区和角缘区的纤维细胞，从底部到顶端逐渐减少。耳蜗线上某一部位的螺旋韧带纤维细胞转运相关酶含量不同，根据免疫组织化学染色的定位和定位，可分为四种类型。血管纹下的I型纤维细胞CA同工酶II、III和CK同工酶BB染色。外沟和螺旋隆突上皮下的II型纤维细胞仅含Na，K-ATPase。位于螺旋韧带下部邻近骨骼的III型纤维细胞含有CA II、III和CK同工酶BB和MM，IV型纤维细胞位于螺旋韧带下部较浅的位置，所有酶的染色程度不同。上工业区浅层纤维细胞表达Na，K-ATPase，而底层纤维细胞表达CA和CK。前庭系统神经感觉上皮下基质板中的大多数纤维细胞含有Na，K-ATPase和CA II，而不含CA III。这些发现提示了上皮细胞与邻近纤维细胞之间在液体和离子转运方面的协同性，并证实了内耳纤维细胞的功能多样性，为螺旋韧带纤维细胞的分类提供了依据。
Fibrocytes in the lateral wall and limbus of the gerbil cochlea evidenced a capacity for ion transport activity by immunostaining for transport mediating enzymes including Na,K-ATPase, carbonic anhydrase (CA) and creatine kinase (CK). Fibrocytes of the spiral ligament unlike those in the suprastrial region and limbus decreased in abundance from base to apex. Spiral ligament fibrocytes at a given position along the cochlea varied in content of transport related enzymes, and on the basis of immunostaining, location and orientation, were classified into four types. Type I fibrocytes under the stria vascularis stained for CA isozymes II and III and CK isozyme BB. Type II fibrocytes under the outer sulcus and spiral prominence epithelium were found to contain only Na,K-ATPase. Type III fibrocytes lying adjacent to bone in the inferior region of the spiral ligament contained CA II and III and CK isozymes BB and MM. Type IV fibrocytes located more superficially in the inferior part of the spiral ligament stained variably for all the enzymes. Superficial fibrocytes in the suprastrial area disclosed Na,K-ATPase whereas the underlying fibrocytes stained for CA and CK. Limbal fibrocytes reacted with antisera to all the enzymes except CA III. Most fibrocytes in stromal plates beneath the vestibular system's neurosensory epithelium contained Na,K-ATPase and CA II but not CA III. These findings point to cooperativity in fluid and ion transport between epithelial cells and neighboring fibrocytes and demonstrate functional diversity of fibrocytes of the inner ear providing a basis for classifying those in the spiral ligament.