Genome-wide identification and characterization of the IPT family members in nine Rosaceae species and a functional analysis of MdIPT5b in cold resistance
Genome-wide identification and characterization of the IPT family members in nine Rosaceae species and a functional analysis of MdIPT5b in cold resistance
复制标题
9种蔷薇科植物IPT家族成员的全基因组鉴定和表征以及MdIPT5b抗寒功能分析
DOI：
10.1016/j.hpj.2022.12.010
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发表时间：
2023-08-01
影响因子：
5.7
通讯作者：
Han，Zhenhai
中科院分区：
文献类型：
--
作者：
Feng，Yi;Lv，Jiahong;Han，Zhenhai
细胞分裂素是参与植物各种生长和发育过程的一组植物激素的成员。异戊烯基转移酶（IPT）是催化细胞分裂素生物合成的限速酶。在本研究中，为了了解 IPT 家族在抗寒性中的作用，在 9 个蔷薇科基因组中鉴定并鉴定了 78 个 IPT 候选者。蔷薇科中IPT家族的扩张主要通过节段复制而不是串联复制发生。一般来说，纯化选择控制着蔷薇科IPT家族的进化，其中IPT3和IPT5同源物是进化的主要驱动力。在MdIPT成员的启动子中发现了参与对许多环境胁迫或植物激素信号的反应的顺式元件。这与苹果愈伤组织中MdIPT基因的表达趋势一致。还发现MdIPT5b对植物激素和应激信号表现出多种反应。 MdIPT5 的异位表达导致转化的苹果愈伤组织和番茄 (Solanum lycopersicum) 幼苗的抗寒性增强。通过冷应激下脯氨酸的积累，氧化还原平衡得到部分稳定。然而，抗坏血酸-谷胱甘肽循环在寒冷中无法稳定。所有生理和生化测定均在分光光度计中进行。这些结果表明，调节IPT基因的表达以适度改善细胞分裂素可以增强脯氨酸的积累，从而稳定渗透和氧化还原平衡，从而提高对冷应激的抵抗力。
Cytokinins are members of a group of phytohormones involved in various growth and developmental processes in plants. Isopentenyl transferase (IPT) is the rate-limiting enzyme in catalyzing the biosynthesis of cytokinins. In this study, to understand the role of IPT family in cold resistance, 78 IPT candidates were identified and characterized in nine Rosaceae genomes. The expansion of IPT families in the Rosaceae primarily occurred through segmental duplication rather than tandem duplication. In general, purifying selection controlled the evolution of IPT families in the Rosaceae, with IPT3 and IPT5 homologs as the primary drivers of evolution.Cis-elements, which are involved in the responses to many environmental stresses or phytohormone signals, were identified in the promoters of MdIPT members. This was consistent with the trends of expression of the MdIPT genes in apple (Malus domestica) calli.MdIPT5bwas also found to exhibit multiple responses to phytohormones and stress signals. The ectopic expression ofMdIPT5bresulted in an increase in cold resistance in transformed apple calli and tomato (Solanum lycopersicum) plantlets. The redox balance was partially stabilized through the accumulation of proline under cold stress. However, the ascorbate–glutathione cycle cannot be stabilized in the cold. All physiological and biochemical assays are preformed in spectrophotometer. These results showed that regulating the expression ofIPTgenes for moderate cytokinin improvement could enhance the accumulation of proline to stabilize the osmotic and redox balances to improve resistance to cold stress.