Synaptonemal complex formation and meiotic checkpoint signaling are linked to the lateral element protein Red1
Synaptonemal complex formation and meiotic checkpoint signaling are linked to the lateral element protein Red1
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DOI：
10.1073/pnas.1004248107
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发表时间：
2010-06-22
影响因子：
11.1
通讯作者：
Jentsch, Stefan
中科院分区：
文献类型：
--
作者：
Eichinger, Christian S.;Jentsch, Stefan
减数分裂从二倍体亲本细胞产生四个单倍体后代。减数分裂的中心步骤是同源染色体的配对和重组，然后在两轮细胞分裂中分离。减数分裂重组由专门的DNA损伤检查点途径监测，并由称为联会复合体（SC）的独特染色体结构指导，但这些事件如何协调尚不清楚。在这里，我们确定SC蛋白Red 1作为早期减数分裂的重要调节因子。Red 1通过两个不同的9- 1 -1亚基特异性基序与9-1 -1检查点复合物的两个亚基相互作用。9-1-1与Red 1的结合不仅对减数分裂检查点的激活而且对SC的形成都是必不可少的。此外，Red 1被SUMO修饰，这促进了Red 1与中心SC元件Zip 1的相互作用，从而确保及时的SC形成。因此，Red 1除了在SC中的结构作用外，还通过将检查点信号传导与SC形成偶联而成为减数分裂的关键协调者。
Meiosis generates four haploid daughters from a diploid parental cell. Central steps of meiosis are the pairing and recombination of homologous chromosomes followed by their segregation in two rounds of cell division. Meiotic recombination is monitored by a specialized DNA damage checkpoint pathway and is guided by a unique chromosomal structure called synaptonemal complex (SC), but how these events are coordinated is unclear. Here, we identify the SC protein Red1 as a crucial regulator of early meiosis. Red1 interacts with two subunits of the 9-1-1 checkpoint complex via two distinct 9-1-1 subunit-specific motifs. Association of 9-1-1 with Red1 is essential not only for meiotic checkpoint activation but for SC formation. Moreover, Red1 becomes SUMO-modified, which fosters interaction of Red1 with the central SC element Zip1, thereby securing timely SC formation. Thus, Red1, in addition to its structural role in the SC, is a crucial coordinator of meiosis by coupling checkpoint signaling to SC formation.