A preparation method for observing intracellular structures by scanning electron microscopy
A preparation method for observing intracellular structures by scanning electron microscopy
复制标题
一种扫描电镜观察细胞内结构的制备方法
作者：
Kéiichi Tanaka;A. Mitsushima
为了用扫描电子显微镜观察细胞内的结构，必须从细胞的断裂表面去除多余的胞质基质。在此之前，我们报道了一种用于此目的的Osmium-DMSO-Osmium方法。这种方法在揭示细胞内结构方面非常有效，但初始固定需要四氧化二臭氧，这会带来一些缺点。本文报道了一种改进的Osmium-DMSO-Osmium方法，该方法用乙醛混合物代替四氧化臭氧作为初始固定剂。由于采用灌注法进行固定，较原方法更好地保存了细微结构，特别是在有死后变性倾向的中枢神经组织中。此外，该方法还可以用扫描电子显微镜(SEM)对细胞内结构进行细胞化学研究。在本研究中，溶酶体的酸性磷酸酶在彩色扫描电子显微镜下被证实。
In order to observe intracellular structures by scanning electron microscopy, excess cytoplasmic matrix must be removed from the fractured surface of cells. Previously we reported an Osmium‐DMSO‐Osmium method devised for this purpose. This method is very effective in revealing intracellular structures, but requires osmium tetroxide for initial fixation with some consequent disadvantages. In the present study, a revised Osmium‐DMSO‐Osmium method is reported, in which an aldehyde mixture is used as the initial fixative instead of osmium tetroxide. As fixation is carried out by perfusion in this revised method, better preservation of fine structures is achieved than by the original method, especially in the central nervous tissue which tends to suffer from post‐mortem degeneration. Moreover this method can be applied to cytochemical studies of intracellular structures with a scanning electron microscope (SEM). In this study, acid phosphatase of lysosomes is demonstrated in a coloured SEM micrograph.
通过白细胞背散射电子成像进行超微结构细胞化学定位。
DOI：
10.1177/29.9.6945361
发表时间：
1981
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Soligo,D;deHarven,E
通讯作者：
deHarven,E