ANALYSIS OF THE COMPLETE NUCLEOTIDE-SEQUENCE OF CHP1, A PHAGE WHICH INFECTS AVIAN CHLAMYDIA-PSITTACI
ANALYSIS OF THE COMPLETE NUCLEOTIDE-SEQUENCE OF CHP1, A PHAGE WHICH INFECTS AVIAN CHLAMYDIA-PSITTACI
复制标题
DOI：
10.1099/0022-1317-70-12-3381
复制
发表时间：
1989-12-01
影响因子：
3.8
通讯作者：
RICHMOND, SJ
中科院分区：
文献类型：
--
作者：
STOREY, CC;LUSHER, M;RICHMOND, SJ
我们报道了噬菌体Chp1的完整核苷酸序列。基因组被发现有4877个碱基长，它可能编码11种蛋白质。开放阅读框(ORF)6和7分别位于ORFs2和ORFs1内，但在第二阅读框中。当将Chp1与EMBL数据库进行比较时，没有发现显著的DNA同源性。测定了3种结构蛋白VP1、VP2和VP3的N末端氨基酸序列，发现它们分别由ORF1、2和3编码。氨基酸同源性研究表明，VP1与Vphi.X174和S13噬菌体的主要结构蛋白具有同源性，从ORF4推测的蛋白与Vphi.X174的A蛋白以及S13和G4的A蛋白同源性。Chp1的基因组具有与vphi.X174相似的组织结构，尽管它的碱基少了509个碱基。我们认为Chp1是微病毒科的一个成员，但它的差异足以证明它是一个亚家族，我们称之为衣原体亚科。
We report the complete nucleotide sequence of bacteriophage Chp1. The genome was found to be 4877 bases long and it potentially codes for 11 proteins. Open reading frames (ORFs) 6 and 7 lie within ORFs2 and 1 respectively but are in a second reading frame. No significant DNA homology was found when Chp1 was compared to the EMBL database. The N-terminal amino acid sequences of the three structural proteins VP1, VP2 and VP3 were determined and it was found that they were encoded by ORFs 1, 2 and 3 respectively. Amino acid homology studies revealed that VP1 has homology with the major structural protein of bacteriophages .vphi.X174 and S13, and that the protein inferred from ORF4 shows homology to the A proteins of .vphi.X174, and S13 and G4. The genome of Chp1 has an organization similar to that of .vphi.X174 although it is 509 bases smaller. We propose that Chp1 is a member of the Microviridae but that it is sufficiently different to warrant its own subfamily which we have called the Chlamydiavirinae.