Conservation of the drought-inducible, DS2 genes and divergences from their ASR paralogues in solanaceous species
Conservation of the drought-inducible, DS2 genes and divergences from their ASR paralogues in solanaceous species
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DOI：
10.1016/j.plaphy.2005.02.002
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发表时间：
2005-03-01
影响因子：
6.5
通讯作者：
Bánfalvi, Z
中科院分区：
文献类型：
--
作者：
Dóczi, R;Kondrák, M;Bánfalvi, Z
马铃薯干旱诱导基因DS 2属于脱落酸、胁迫和成熟基因家族。以前的研究表明，DS 2基因的表达在马铃薯叶片中是高度脱水特异性的，不受冷、热、盐、缺氧或氧化胁迫的诱导，并且不依赖于脱落酸（阿坝）。现在表明StDS 2对蔗糖或任何植物激素都没有反应。在与马铃薯具有不同亲缘关系的茄科物种中，研究了具有这种独特调控模式的DS 2基因的保守性。DS 2的直系同源物被确定在密切相关的番茄和辣椒属物种的DNA序列比对，但不是在更远的相关烟草sp. DNA和RNA凝胶印迹分析显示，存在一个基因高度同源的马铃薯基因StDS 2在番茄（LeDS 2）具有相同的干燥特异性表达在叶片和器官特异性表达在花和绿色水果。LeDS 2启动子被分离出来，并发现与StDS 2的启动子序列几乎相同，除了在番茄中插入了45-bp。与此相反，没有基因高度相似的StDS 2检测烟草物种的DNA凝胶印迹。StDS 2和LeDS 2启动子区均不能使转基因烟草植物中的β-葡萄糖醛酸酶（GUS）报告基因表达，表明DS 2表达所需的反式调节因子在烟草中也不保守。这些数据表明，一个狭窄的种特异性和后期进化的DS 2型基因在茄科。(c)2005年，Elsevier SAS。All rights reserved.
The drought-inducible DS2 genes of potatoes are members of the ASR (abscisic acid, stress and ripening) gene family. Previously it was shown that expression of DS2 genes is highly dehydration-specific in potato leaves, is not inducible by cold, heat, salt, hypoxia or oxidative stresses, and is independent of abscisic acid (ABA). Now it is shown that StDS2 does not respond either to Sucrose or any plant hormones. Conservation of DS2 genes with this unique mode of regulation was Studied in the solanaceous species with different relationships to potatoes. DS2 orthologues were identified by DNA sequence alignment in the closely related Lycopersicon and Capsicum species but not in the more distantly related Nicotiana sp. DNA and RNA gel blot analysis revealed the presence of a gene highly homologous to the potato gene StDS2 in tomato (LeDS2) with the same desiccation-specific expression in leaves and organ-specific expression in flowers and green fruits. The LeDS2 promoter was isolated and found to be almost identical in sequence with the promoter of StDS2, except for a 45-bp insertion in tomato. In contrast, no gene highly similar to StDS2 was detected in Nicotiana species on DNA gel blots. Neither StDS2 nor LeDS2 promoter regions were able to confer expression for the beta-glucuronidase (GUS) reporter gene in transgenic tobacco plants indicating that the trans regulatory factors necessary for DS2 expression are not conserved either in Nicotiana tabacum. These data suggest a narrow species-specificity and late evolution of the DS2-type genes within the family Solanaceae. (c) 2005 Elsevier SAS. All rights reserved.