IMPROVED PRESERVATION OF ULTRASTRUCTURE IN DIFFICULT-TO-FIX ORGANISMS BY HIGH-PRESSURE FREEZING AND FREEZE SUBSTITUTION .1. DROSOPHILA-MELANOGASTER AND STRONGYLOCENTROTUS-PURPURATUS EMBRYOS
IMPROVED PRESERVATION OF ULTRASTRUCTURE IN DIFFICULT-TO-FIX ORGANISMS BY HIGH-PRESSURE FREEZING AND FREEZE SUBSTITUTION .1. DROSOPHILA-MELANOGASTER AND STRONGYLOCENTROTUS-PURPURATUS EMBRYOS
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DOI：
10.1002/jemt.1070240603
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发表时间：
1993-04-15
影响因子：
2.5
通讯作者：
MORPHEW, MK
中科院分区：
文献类型：
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作者：
MCDONALD, K;MORPHEW, MK
在这项研究中，我们已经应用了高压冷冻和冷冻替代技术，胚胎细胞类型，通常很难固定正确的电子显微镜。在果蝇和圆海胆purpuratus，我们看到改进的保护膜系统和细胞骨架相比，发表的结果相同的细胞使用传统的电子显微镜（EM）固定方法。最后，我们已经看到，即使在冷冻置换过程中轻微锇固定后，切片的植入后标记也是可能的。
In this study, we have applied the techniques of high pressure freezing and freeze substitution to embryonic cell types which are usually difficult to fix properly for electron microscopy. In both Drosophila and Strongylocentrotus purpuratus, we see improved preservation of both membrane systems and cytoskeleton when compared to published results on the same cells using conventional electron microscope (EM) fixation methods. Finally, we have seen that postembedding labelling of sections is possible even after light osmium fixation during freeze substitution.