ISOLATION OF THE CAUSATIVE AGENT OF HANTAVIRUS PULMONARY SYNDROME
ISOLATION OF THE CAUSATIVE AGENT OF HANTAVIRUS PULMONARY SYNDROME
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DOI：
10.4269/ajtmh.1994.51.102
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发表时间：
1994-07-01
影响因子：
3.3
通讯作者：
PETERS, CJ
中科院分区：
文献类型：
--
作者：
ELLIOTT, LH;KSIAZEK, TG;PETERS, CJ
对最近在美国西南部爆发的急性呼吸道疾病的调查发现了一种新的疾病，汉坦病毒肺综合征（HPS），具有高死亡率。不同的动物和细胞系被用于尝试分离病原体。一种以前未知的汉坦病毒在实验室饲养的鹿鼠中传代，从鹿鼠（Peromyscus maniculatus）的肺组织中回收，并在Vero细胞的E6克隆中繁殖。通过间接免疫荧光试验，使用HPS患者的恢复期血清，很容易在感染的细胞中检测到病毒抗原。经电镜观察，该病毒具有布尼亚病毒科汉坦病毒属成员的典型形态特征。病毒序列对应于先前检测到的巢式逆转录酶-聚合酶链反应检测汉坦病毒感染的标本从啮齿动物和人类。这种新发现的病毒是HPS的病原体，暂命名为Muerto峡谷病毒。
Investigation of a recent outbreak of acute respiratory illness in the southwestern United States resulted in the recognition of a new disease, hantavirus pulmonary syndrome (HPS) with high mortality. Different animals and cell lines were used in attempts to isolate the causative agent. A previously unknown hantavirus was passaged in laboratory-bred deer mice, recovered from lung tissues of a deer mouse, Peromyscus maniculatus, and propagated in the E6 clone of Vero cells. Virus antigen was readily detected in the infected cells by an indirect immunofluorescence assay, using convalescent-phase sera from HPS patients. By electron microscopy, the virus was shown to have the typical morphologic features of members of the genus Hantavirus, family Bunyaviridae. Virus sequences corresponded to those previously detected by a nested reverse transcriptase-polymerase chain reaction assay of hantavirus-infected specimens from rodents and humans. This newly recognized virus, the etiologic agent of HPS, has been tentatively named Muerto Canyon virus.