The Protective Effects of Apocynin on Kidney Damage Caused by Renal Ischemia/Reperfusion
The Protective Effects of Apocynin on Kidney Damage Caused by Renal Ischemia/Reperfusion
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DOI：
10.1089/end.2012.0556
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发表时间：
2013-05-01
影响因子：
2.7
通讯作者：
Parlakpinar, Hakan
中科院分区：
文献类型：
--
作者：
Altintas, Ramazan;Polat, Alaadin;Parlakpinar, Hakan
目的：本实验旨在探讨NADPH氧化酶抑制剂夹竹桃麻素对大鼠缺血再灌注（I/R）肾损伤的保护作用。方法：将32只大鼠随机分为对照组和3个I/R组（缺血1小时，再灌注23小时）。三个 I/R 组在两个不同时间点（缺血前和缺血期间）用罗布麻宁（20 mg/kg，腹腔注射）治疗。组织病理学结果，包括细胞凋亡变化以及组织丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPX）、还原型谷胱甘肽（GSH）、髓过氧化物酶（MPO）、血尿素氮（BUN）和血清肌酐（Cr）水平， 结果:I/R组肾组织MDA、MPO、血清BUN、Cr含量显着升高,但SOD、CAT、GPX、GSH含量与对照组比较差异无统计学意义。虽然与 I/R 组相比，罗布麻宁显着降低了第 3 组的 MDA 和 MPO，并增加了两个治疗组的 GPX，但治疗组中升高的 BUN 和 Cr 水平显着降低。在组织学评估中，肾缺血再灌注损伤还引起广泛的肾小管坏死、肾小球损伤和细胞凋亡。罗布麻宁，尤其是在缺血期间使用时，在治疗组中不同程度地改善了这些组织学损伤。 结论：首次评估了罗布麻宁对肾缺血再灌注损伤的有益作用。
Purpose: This experimental study was designed to explore the protective effect of apocynin, the NADPH-oxidase inhibitor, on kidney damage induced by ischemia/reperfusion (I/R) in a rat model.Methods: Thirty-two rats were randomly divided into a control group and three I/R groups (1-hour ischemia followed by 23-hour reperfusion). Three I/R groups were treated by apocynin (20 mg/kg, i.p.) at two different time points (before ischemia and during ischemia). The histopathological findings, including apoptotic changes, and also tissue malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathion peroxidase (GPX), reduced glutathione (GSH), myeloperoxidase (MPO), blood urea nitrogen (BUN), and serum creatinine (Cr) levels, were determined.Results: Kidney tissue MDA and MPO, and serum BUN and Cr levels were found to be significantly higher in the I/R group, but there was no statistically significant difference in the levels of SOD, CAT, GPX, and GSH between the I/R and the control groups. Although apocynin significantly reduced MDA and MPO in group 3 and increased GPX in both treatment groups when compared to the I/R group, the elevated BUN and Cr levels were significantly reduced in treatment groups. Renal I/R injury also induced extensive tubular necrosis, glomerular damage, and apoptosis in the histological evaluation. Apocynin, especially when used during ischemia, ameliorated these histological damages in different amounts in treatment groups.Conclusion: The beneficial effects of apocynin on renal I/R injury were evaluated for the first time.