Quantification and antioxidant and anti-HCV activities of the constituents from the inflorescences of Scabiosa comosa and S. tschilliensis
Quantification and antioxidant and anti-HCV activities of the constituents from the inflorescences of Scabiosa comosa and S. tschilliensis
复制标题
Scabiosa comosa 和 S. tschilliensis 花序成分的定量及其抗氧化和抗 HCV 活性
DOI：
10.1080/14786419.2015.1027701
复制
发表时间：
2016-01-01
影响因子：
2.2
通讯作者：
Ma, C. M.
中科院分区：
文献类型：
--
作者：
Ma, J. N.;Bolraa, S.;Ma, C. M.
为了研究传统上用于治疗肝病的鸡冠花序中的生物活性成分，我们用2，2‘-二苯基-1-苦基肼(DPPH)、2，2’-azinobis-(3-ethylbenzthiazoline-6-sulphonic酸(ABTS)、铁还原抗氧化剂(FRAP)和DPPH-超高效液相色谱-质谱仪(UPLC-MS)测定了它们的抗氧化活性。此外，对主要化合物的细胞抗丙型肝炎病毒活性进行了评价。植物提取物具有较强的抗氧化活性。3，4-二咖啡酰奎宁酸(DCQA)、3，5-DCQA和4，5-DCQA均为首次从SCabiosa属植物中分离得到。建立了多反应监测(MRM)模式下的超高效液相色谱-质谱法(UPLC-MS)，对花序中的18种化学成分进行了定量测定。3，5-DCQA、绿原酸和木犀草素或芹菜素的一些糖苷是含量最丰富的成分。绿原酸和3，5-DCQA具有良好的清除自由基活性和抗丙型肝炎病毒的活性。这些发现为该中药临床应用于肝病提供了科学依据。
To investigate the bioactive constituents of the inflorescences of Scabiosa comosa and S. tschilliensis, which are used traditionally for liver diseases, we tested the antioxidant activity using 2,2'-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), ferric reducing antioxidant potential (FRAP), and DPPH-ultra high performance liquid chromatography-mass spectrometer (UPLC-MS) assay. In addition, cell-based anti-HCV activity of the major compounds were evaluated. The plant extracts showed strong antioxidant activity. For the first time, 3,4-dicaffeoylquinic acid (DCQA), 3,5-DCQA and 4,5-DCQA were identified from genus Scabiosa. A UPLC-MS method in multiple reaction monitoring (MRM) mode was established to quantify 18 constituents in the inflorescences of Scabiosa. The 3,5-DCQA, chlorogenic acid and some glycosides of luteolin or apigenin were found to be the most abundant constituents. Chlorogenic acid and 3,5-DCQA showed excellent radical scavenging activity and demonstrated anti-HCV activity. These findings provided scientific evidences for the clinic use of this herbal medicine for liver diseases.