Mangiferin inhibited neuroinflammtion through regulating microglial polarization and suppressing NF-κB, NLRP3 pathway
Mangiferin inhibited neuroinflammtion through regulating microglial polarization and suppressing NF-κB, NLRP3 pathway
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芒果苷通过调节小胶质细胞极化和抑制NF-κB、NLRP3途径抑制神经炎性反应
DOI：
10.1016/s1875-5364(21)60012-2
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发表时间：
2021-02-25
影响因子：
4.6
通讯作者：
Tang Zhi-Shu
中科院分区：
文献类型：
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作者：
Lei Li-Yan;Wang Rui-Cheng;Tang Zhi-Shu
炎症在神经退行性疾病（例如帕金森病和阿尔茨海默病）的进展中起着重要作用。小胶质细胞负责中枢神经系统（CNS）的稳态，并参与神经炎症。因此，抑制小胶质细胞介导的神经炎症可能具有治疗神经退行性疾病的潜力。芒果苷是知母中氧杂蒽酮的主要苷，具有抗炎、抗糖尿病和抗氧化特性。然而，芒果苷对小胶质细胞炎症反应的影响仍知之甚少。在本研究中，我们研究了芒果苷抑制 LPS 诱导的 BV2 小胶质细胞炎症的机制。 BV2细胞用芒果苷预处理，然后进行LPS刺激。体外测定中，对 NO 和细胞因子的产生进行了定量。采用Western blot和免疫细胞化学检测芒果苷对BV2细胞极化及信号通路的影响。结果表明，芒果苷治疗显着减少NO、IL-1β、IL-6和TNF-α的产生，还减少iNOS和COX-2的mRNA和蛋白，促进炎症向抗炎极化，并抑制NF-κB和NLRP3炎症小体的激活。这些数据表明，芒果苷通过调节小胶质细胞巨噬细胞极化和抑制 NF-kappa B 和 NLRP3 信号通路而具有抗神经炎症特性，并可能作为神经炎症疾病的潜在天然治疗候选者。
Inflammation plays important roles in the progress of neurodegenerative diseases, such as Parkinson's disease and Alzheimer's disease. Microglia is responsible for the homeostasis of the central nervous system (CNS), and involved in the neuroinflammation. Therefore, it could be potential in treatment of neurodegenerative diseases to suppress the microglia-mediated neuroinflammation. Mangiferin, a major glucoside of xanthone in Anemarrhena Rhizome, has anti-inflammatory, anti-diabetes, and anti-oxidative properties. However, the effect of mangiferin on the inflammatary responses of microglia cells are still poorly understand. In this study, we investigated the mechanism by which mangiferin inhibited inflammation in LPS-induced BV2 microglia cells. BV2 cells were pretreatment with mangiferin followed by LPS stimulation. In vitro assays, NO and cytokines production were quantified. Western blot and immunocytochemistry were used to examine the effect of mangiferin on the polarization of BV2 cells and signaling pathway. The results showed that mangiferin treatment significantly reduced NO, IL-1 beta, IL-6 and TNF-alpha production, also reduced the mRNA and protein of iNOS and COX-2, promoted the polarization of inflammatory toward anti-inflammatory, and inhibited activation of NF-kappa B and NLRP3 inflammasome. These data suggest that mangiferin has an anti-neuroinflammatory property via regulating microglia macrophage polarization and suppressing NF-kappa B and NLRP3 signaling pathway, and may act as a potential natural therapeutic candidate for neuroinflammatory diseases.