The ETS-transcription factor Pointed is sufficient to regulate the posterior fate of the follicular epithelium
The ETS-transcription factor Pointed is sufficient to regulate the posterior fate of the follicular epithelium
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DOI：
10.1242/dev.189787
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发表时间：
2020-10
期刊：
影响因子：
4.6
通讯作者：
Cody A. Stevens;Nicole T. Revaitis;Rumkan Caur;N. Yakoby
中科院分区：
文献类型：
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作者：
Cody A. Stevens;Nicole T. Revaitis;Rumkan Caur;N. Yakoby
摘要 Janus 激酶/信号转导和转录激活剂 (JAK/STAT) 通路调节果蝇滤泡细胞的前后轴。在前部，它通过 BMP 配体 decapentaplegic (dpp) 的表达激活骨形态发生蛋白 (BMP) 信号通路。在后部，JAK/STAT 与表皮生长因子受体 (EGFR) 通路一起表达 T-box 转录因子中线 (mid)。尽管 MID 对于确定卵室的后命运是必要的，但我们表明它不足以确定后命运。 ETS 转录因子点 (pnt) 在滤泡细胞中的重叠区域中表达。本研究表明pnt位于mid的上游，足以在前端诱导后命运，其特点是诱导mid，防止拉伸细胞形成和废除边界细胞迁移。我们证明前部 BMP 信号传导被 PNT 通过 dpp 抑制而消除。然而，异位 DPP 无法挽救前命运的形成，表明 PNT 的其他靶点参与了后命运的决定。摘要：转录因子 Pointed 调节后决定簇中线；它足以抑制 BMP 信号传导并消除前滤泡上皮中的所有细胞形态发生。
ABSTRACT The Janus-kinase/signal transducer and activator of transcription (JAK/STAT) pathway regulates the anterior posterior axis of the Drosophila follicle cells. In the anterior, it activates the bone morphogenetic protein (BMP) signaling pathway through expression of the BMP ligand decapentaplegic (dpp). In the posterior, JAK/STAT works with the epidermal growth factor receptor (EGFR) pathway to express the T-box transcription factor midline (mid). Although MID is necessary for establishing the posterior fate of the egg chamber, we show that it is not sufficient to determine a posterior fate. The ETS-transcription factor pointed (pnt) is expressed in an overlapping domain to mid in the follicle cells. This study shows that pnt is upstream of mid and that it is sufficient to induce a posterior fate in the anterior end, which is characterized by the induction of mid, the prevention of the stretched cells formation and the abrogation of border cell migration. We demonstrate that the anterior BMP signaling is abolished by PNT through dpp repression. However, ectopic DPP cannot rescue the anterior fate formation, suggesting additional targets of PNT participate in the posterior fate determination. Summary: The transcription factor Pointed regulates the posterior determinant Midline; it is sufficient to repress BMP signaling and to abrogate all cell morphogenesis in the anterior follicular epithelium.