Crown ether-mediated extraction and functional conversion of cytochrome c in ionic liquids
Crown ether-mediated extraction and functional conversion of cytochrome c in ionic liquids
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DOI：
10.1021/bm050847t
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发表时间：
2006-01-01
影响因子：
6.2
通讯作者：
Goto, M
中科院分区：
文献类型：
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作者：
Shimojo, K;Nakashima, K;Goto, M
我们报告说，大环配体使蛋白质从水相转移到离子液体。研究了离子液体对血红素蛋白-细胞色素c（Cyt-c）的萃取行为。一种含羟基的离子液体与二环己基-18-冠-6被发现能够定量分配的Cyt-c，而使用常规的有机溶剂的蛋白质转移是微不足道的小。此外，我们澄清了Cyt-c溶解在离子液体中引起的Cyt-c的结构转变，这触发其功能转换从电子转移蛋白过氧化物酶。
We report that a macrocyclic ligand enables transfer of a protein from an aqueous phase to ionic liquids. The extraction behavior of heme protein cytochrome c (Cyt-c) from an aqueous phase into ionic liquids was investigated with crown ethers. A hydroxyl-group-containing ionic liquid with dicyclohexano-18-crown-6 was found to be capable of quantitative partitioning of Cyt-c, whereas the protein transfer using conventional organic solvents was negligibly small. Furthermore, we clarified that Cyt-c solubilized in ionic liquids caused a structural transformation of Cyt-c, which triggers its functional conversion from an electron-transfer protein to peroxidase.