CRYSTALS IN CALCIFIED EPIPHYSEAL CARTILAGE AND CORTICAL BONE OF THE RAT
CRYSTALS IN CALCIFIED EPIPHYSEAL CARTILAGE AND CORTICAL BONE OF THE RAT
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DOI：
10.1007/bf02555138
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发表时间：
1988-10-01
影响因子：
4.2
通讯作者：
GRYNPAS, MD
中科院分区：
文献类型：
--
作者：
ARSENAULT, AL;GRYNPAS, MD
研究了大鼠钙化软骨和皮质骨中羟基磷灰石（HAP）晶体的大小，并与人工合成的低结晶羟基磷灰石（PCHA）进行了比较。采用x射线衍射法和选择区域暗场成像法测定晶体尺寸，发射光谱法测定元素组成。软骨、骨和PCHA的晶体大小在120 - 170 . ang之间。长度增加了50。宽度由x射线衍射和暗场成像确定。软骨灰分重低于骨灰分重，但钙磷比相同，均为1.6。这些发现虽然与x射线衍射文献一致，但与传统的亮场电子显微镜观察结果不同。我们得出结论，软骨和骨中的矿物位点，以板状结构存在，实际上是小的120-170倍的聚集体。50 .ANG。HAP晶体。从晶体与胶原蛋白和其他大分子的关系的角度讨论了这些发现的后果。
The size of hydroxyapatite (HAP) crystals in calcified cartilage and cortical bone of the rat has been studied and compared with that of synthetic poorly crystalline hydroxyapatite (PCHA). Crystal size was determined by X-ray diffraction and selected-area dark field imaging, and their elemental compostions were determined by emission spectroscopy. The crystal size of cartilage, bone, and PCHA were found to be between 120 and 170 .ANG. in length by 50 .ANG. in width as determined by both X-ray diffraction and dark field imaging. Cartilage had a lower ash weight than bone but both have the same Ca/P ratio of 1.6. These findings, though in agreement with the X-ray diffraction literature, differ from observations made by conventional bright field electron microscopy. We conclude that minerial sites in cartilage and bone, which exist as platelike structures, are in fact aggregates of small 120-170 .times. 50 .ANG. HAP crystals. The consequences of these findings are discussed in view of crystal relationships with collagen and other macromolecules.