Chronic food restriction enhances dopamine-mediated intracranial self-stimulation.
Chronic food restriction enhances dopamine-mediated intracranial self-stimulation.
复制标题
慢性食物限制增强多巴胺介导的颅内自我刺激。
DOI：
10.1097/wnr.0000000000001700
复制
发表时间：
2021-09-08
期刊：
影响因子：
1.7
通讯作者：
Beeler JA
中科院分区：
文献类型：
--
作者：
Gnazzo FG;Mourra D;Guevara CA;Beeler JA
多巴胺（DA）介导的强化和行为适应对生存至关重要。在这里，我们使用光学颅内自我刺激（oICSS）测试食物限制对DA介导的学习和强化的影响，oICSS是传统电ICSS（也称为脑刺激奖励，BSR）的光遗传学版本。使用小鼠遗传系选择性地表达通道视紫红质在中脑DA神经元，我们表明，基因表达的通道视紫红质可以介导光学诱发的多巴胺释放和支持自我刺激的压下范式。使用这种中脑多巴胺oICSS制剂，我们比较了随意与食物限制小鼠的获取和按压速率。食物限制促进更快的收购自我刺激行为和更高的反应率;收购后逆转食物状态调制反应活力在已经建立的行为。这些数据表明，食物限制提高收购和表达多巴胺增强自我刺激反应。这些数据表明，oICSS的效用，检查伴随着DA信号的实验操作，如食物限制诱导的神经适应强化学习的变化。
Dopamine (DA) mediated reinforcement and behavioral adaptation is essential to survival. Here we test the effects of food restriction on DA mediated learning and reinforcement using optical intracranial self-stimulation (oICSS), an optogenetic version of conventional electrical ICSS (also known as Brain Stimulation Reward, BSR). Using mouse genetic lines to express channelrhodopsin selectively in midbrain DA neurons, we demonstrate that genetically expressed channelrhodopsin can mediate optically evoked dopamine release and support self-stimulation in a lever-pressing paradigm. Using this midbrain dopamine oICSS preparation, we compare acquisition and rate of pressing in ad libitum versus food restricted mice. Food restriction facilitated both more rapid acquisition of self-stimulation behavior and higher rates of responding; reversing food status after acquisition modulated response vigor in already established behavior. These data suggest that food restriction enhances both the acquisition and expression of dopamine-reinforced self-stimulation responding. These data demonstrate the utility of oICSS for examining changes in reinforcement learning concomitant to neuroadaptations induced in DA signaling by experimental manipulations such as food restriction.