ADRIAMYCIN, 14-HYDROXYDAUNOMYCIN, A NEW ANTITUMOR ANTIBIOTIC FROM S-PEUCETIUS VAR CAESIUS
ADRIAMYCIN, 14-HYDROXYDAUNOMYCIN, A NEW ANTITUMOR ANTIBIOTIC FROM S-PEUCETIUS VAR CAESIUS
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DOI：
10.1002/bit.260110607
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发表时间：
1969-01-01
影响因子：
3.8
通讯作者：
SPALLA, C
中科院分区：
文献类型：
--
作者：
ARCAMONE, F;CASSINEL.G;SPALLA, C
该链霉菌是从S.通过诱变处理，产生柔红霉素的微生物peucetius与亲本培养物的区别在于营养菌丝体和气生菌丝体的颜色以及其抗生素产生能力。在摇瓶中和搅拌的发酵罐中，在含有葡萄糖、啤酒酵母和无机盐的培养基上，在浸没和通气培养物中积累阿霉素。通过溶剂萃取、缓冲纤维素柱色谱法和盐酸盐结晶进行产物分离。新型抗肿瘤药物阿霉素是道诺霉素的14-羟基衍生物。
Streptomyces peucetiusvar.caesius, obtained fromS. peucetius, the daunomycin producing microorganism, by mutagenic treatment, differs from the parent culture by the color of the vegetative and aerial mycelia and by its antibiotic, producing ability.S. peucetiusvar.caesiusaccumulates adriamycin in submerged and aerated culture on a medium containing glucose, brewer's yeast, and inorganic, salts both in shake flasks and in stirred fementers. Isolation of the product is performed by solvent extraction, chromatography on buffered cellulose columns, and crystallization as the hydrochloride. The new antitumor agent, adriamycin, is the 14‐hydroxv derivative of daunomyein.