Interactions of troponin subunits: free energy of binary and ternary complexes.
Interactions of troponin subunits: free energy of binary and ternary complexes.
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肌钙蛋白亚基的相互作用：二元和三元复合物的自由能。
DOI：
10.1021/bi00392a049
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Malik,NA
中科院分区：
文献类型：
--
作者：
Cheung,HC;Wang,CK;Malik,NA
材料和方法肌钙蛋白如前所述从兔骨骼肌中分离（Cheung等，1982年）。它的三个亚基在6种尿素中解离，并在尿素存在下通过柱层析分离（Wang & Cheung，1986）。TnC获自Whatman DE-52柱，TnI获自Whatman DE-52柱。
Materials and MethodsTroponin was isolated from rabbit skeletal muscle as previously described (Cheung et al., 1982). Its three subunits were dissociated in 6 urea and separated by column chromatography in thepresence of urea(Wang & Cheung, 1986). TnC was obtained from a Whatman DE-52 column, and Tnl