ISOLATION OF EXTRACHROMOSOMAL DEOXYRIBONUCLEIC ACIDS FROM EXTREMELY THERMOPHILIC BACTERIA
ISOLATION OF EXTRACHROMOSOMAL DEOXYRIBONUCLEIC ACIDS FROM EXTREMELY THERMOPHILIC BACTERIA
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DOI：
10.1099/00221287-104-2-193
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发表时间：
1978-01-01
期刊：
影响因子：
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通讯作者：
SAKAGUCHI, K
中科院分区：
文献类型：
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作者：
HISHINUMA, F;TANAKA, T;SAKAGUCHI, K
采用氯化铯-溴化乙啶密度梯度离心法检测菌株(8)中共价闭合环状DNA分子的存在。其中4株黄热菌BS1、AT61、AT62和嗜热热菌HB8携带共价封闭的环状DNA分子。黄曲霉BS1含有2种质粒，分子量为6.1倍。106和17.0。嗜热T. HB8、黄T. AT61和黄T. AT62携带的质粒分子量为6.2倍。106 6.6倍。106倍和6.6倍。106年,分别。用不同的[HindIII， HpaI, HindII， BamNI和SamR]限制性内切酶酶切后，黄T. AT61和AT62质粒在琼脂糖或丙烯酰胺凝胶中的电泳模式无法区分。这是在极端嗜热细菌中存在质粒的第一个证据，尽管它们的功能尚不清楚。[这些质粒可以作为构建重组DNA分子的载体]。
Strains (8) of thermophilic bacteria were examined for the presence of covalently closed circular DNA molecules by CsCl-ethidium bromide density gradient centrifugation. Four of the 8 strains tested, Thermus flavus BS1, AT61, AT62 and T. thermophilus HB8 carried covalently closed circular DNA molecules. T. flavus BS1 harbored 2 species of plasmids with MW of 6.1 .times. 106 and 17.0 .times. 106 as determined by EM. T. thermophilus HB8, T. flavus AT61 and T. flavus AT62 carried plasmids with MW 6.2 .times. 106, 6.6 .times. 106 and 6.6 .times. 106, respectively. Plasmids from T. flavus AT61 and AT62 were indistinguishable in their electrophoretic patterns in agarose or acrylamide gel after digestion with various [HindIII, HpaI, HindII, BamNI and SamR] restriction endonucleases. This is the 1st evidence for the presence of plasmids in extremely thermophilic bacteria, though their functions are unknown. [These plasmids may serve as vectors for the construction of recombinant DNA molecules].