Ovarian and hormonal responses to single or continuous peripheral administration of senktide, a neurokinin 3 receptor agonist, during the follicular phase in goats
Ovarian and hormonal responses to single or continuous peripheral administration of senktide, a neurokinin 3 receptor agonist, during the follicular phase in goats
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DOI：
10.1016/j.domaniend.2015.07.003
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发表时间：
2015-10-01
影响因子：
2.1
通讯作者：
Tanaka, T.
中科院分区：
文献类型：
--
作者：
Endo, N.;Rahayu, L. P.;Tanaka, T.
本研究旨在探讨单次或连续给予神经激肽3受体激动剂senktide对卵泡期山羊激素和卵泡动力学的影响。在黄体期给山羊注射PGF(2 α)，然后用阴道内黄体酮装置治疗10 d。停止黄体酮治疗12 h后，山羊接受单次静脉注射senktide （200 nmol, n = 4）或对照（n = 4），或连续静脉注射senktide （20 nmol/min, 20 nmol/min）。n = 6)或对照（n = 6），持续6小时。实验期间进行血样和卵巢超声检查。单次注射senktide对黄体生成素（LH）脉冲数和平均LH浓度没有影响。另一方面，连续注射senktide导致黄体生成素分泌持续增加，在开始注射后6 h，每隔10 min采集样品的平均黄体生成素浓度高于对照组（2.8 +/- 1.3 vs 1.0 +/- 0.6 ng/mL, P < 0.01）。在6只山羊中，有4只山羊的LH浓度在注射senktide后6小时达到峰值，在注射后48小时排卵，无发情行为。其余2只经senktide治疗的山羊和所有经车辆治疗的山羊在治疗后72和96 h均出现了发情和排卵。这些结果表明，在卵泡期山羊持续输注senktide可使LH持续升高，并提前排卵时间。(C) 2015爱思唯尔公司版权所有。
The present study aimed to investigate the effects of single or continuous administration of a neurokinin 3 receptor agonist, senktide, on hormonal and follicular dynamics in follicular phase goats. Goats were injected with PGF(2 alpha) in the luteal phase and treated with an intravaginal progesterone device for 10 d. At 12 h after the cessation of progesterone treatment, the goats received a single intravenous injection of senktide (200 nmol, n = 4) or vehicle (n = 4), or continuous intravenous infusion of senktide (20 nmol/min, n = 6) or vehicle (n = 6) for 6 h. Blood sampling and ovarian ultrasonography were performed during the experiment A single injection of senktide did not influence the number of luteinizing hormone (LH) pulses and mean LH concentration. On the other hand, continuous injection of senktide caused a sustained increase in LH secretion, and mean LH concentration in samples collected at 10-min intervals for 6 h after the start of infusion was higher than that of vehicle-treated goats (2.8 +/- 1.3 vs 1.0 +/- 0.6 ng/mL, P < 0.01). In 4 of 6 goats, LH concentrations reached their peaks during the 6-h senktide infusion, and ovulation was observed at 48 h after the start of infusion without estrous behavior. The remaining 2 senktide-treated goats and all vehicle-treated goats showed estrus and ovulated at 72 or 96 h after treatment These results suggest that continuous infusion of senktide in follicular phase goats can cause a sustained increase in LH and advance the time of ovulation. (C) 2015 Elsevier Inc. All rights reserved.