Live-Cell Fluorescence Imaging of Magnetosome Organelle for Magnetotaxis Motility
Live-Cell Fluorescence Imaging of Magnetosome Organelle for Magnetotaxis Motility
复制标题
磁小体细胞器的活细胞荧光成像用于趋磁运动
DOI：
10.1007/978-1-0716-3060-0_12
复制
发表时间：
2023
影响因子：
--
通讯作者：
Taoka Azuma
中科院分区：
文献类型：
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作者：
Eguchi Yukako;Taoka Azuma
细胞内动力学的评估对于理解细菌细胞器的功能和形成过程是至关重要的，就像它对于研究真核生物的细胞器一样。本章介绍了利用高倾斜叠层光学薄片(HILO)显微镜对趋磁细菌细胞中的磁小体细胞器进行荧光成像的方法。此外，作为荧光磁小体成像的一种应用，我们还介绍了磁小体管腔内pH成像的方法。
The assessment of intracellular dynamics is crucial for understanding the function and formation process of bacterial organelle, just as it is for the inquisition of their eukaryotic counterparts. The methods for imaging magnetosome organelles in a magnetotactic bacterial cell using live-cell fluorescence imaging by highly inclined and laminated optical sheet (HILO) microscopy are presented in this chapter. Furthermore, we introduce methods for pH imaging in magnetosome lumen as an application of fluorescence magnetosome imaging.