Fungicide-driven alterations in azole-resistant Aspergillus fumigatus are related to vegetable crops in Colombia, South America
Fungicide-driven alterations in azole-resistant Aspergillus fumigatus are related to vegetable crops in Colombia, South America
复制标题
DOI：
10.1080/00275514.2018.1557796
复制
发表时间：
2019-03-04
期刊：
影响因子：
2.8
通讯作者：
Le Pape, Patrice
中科院分区：
文献类型：
--
作者：
Alvarez-Moreno, Carlos;Lavergne, Rose-Anne;Le Pape, Patrice
烟曲霉对唑类药物一线治疗耐药的报道见于唑类药物初治患者。这种全球性的耐药性现象与cyp 51 A基因及其启动子区域（如TR 34/L98 H和TR 46/Y121 F/T289 A）的杀菌剂驱动的改变有关。抗唑A.最近在哥伦比亚报道了与在花田中使用三唑类杀菌剂有关的烟曲霉病。本研究的目的是调查是否存在唑类耐药A。从蔬菜作物如胡萝卜、土豆、玉米、草莓和豌豆的土壤样品中，以及从波哥大市周围准备好的农田中，发现了烟曲霉菌。种属鉴定是基于微管蛋白和钙调蛋白基因的测序。全是A。在补充有伊曲康唑或伏立康唑的琼脂上筛选烟曲霉菌株的唑类抗性。在60份土壤样品中，有34份（56.6%）A. 15个样品显示菌株（n = 18）生长在补充有伊曲康唑或伏立康唑的琼脂上。从胡萝卜、马铃薯、玉米和豌豆作物的土壤样品中分离出三唑抗性菌株。cyp 51 A基因及其启动子区的测序显示多态性，主要存在TR 46/Y121 F/T289 A（n = 8）、TR 34/L98 H和TR 53。8个耐药菌株表现出cyp 51 A野生型的启动子区没有改变。我们的研究显示耐唑A.烟曲霉，具有高度的遗传多样性，在哥伦比亚的蔬菜作物。这些数据强调需要确定唑类耐药的流行在A。在哥伦比亚其他地区以及拉丁美洲的临床和环境环境中使用烟曲霉。
Aspergillus fumigatus resistant to azole as first-line therapy has been reported in azole-naive patients. This worldwide resistance phenomenon has been linked to fungicide-driven alterations in the cyp51A gene and its promoter region (such as TR34/L98H and TR46/Y121F/T289A). Azole-resistant A. fumigatus related to the use of triazole fungicides in flower fields was recently reported In Colombia. The purpose of this study was to investigate the presence of azole-resistant A. fumigatus in soil samples from vegetable crops such as carrots, potatoes, maize, strawberries, and pea, and from prepared farming land surrounding the city of Bogota. Species identification was based on sequencing of the -tubulin and calmodulin genes. All A. fumigatus strains were screened for azole resistance on agar supplemented with itraconazole or voriconazole. Among the 60 soil samples, 34 (56.6%) were positive for A. fumigatus and 15 samples exhibited strains (n = 18) that grew on agar supplemented with itraconazole or voriconazole. Triazole-resistant strains were isolated from soil samples associated with carrot, potato, maize, and pea crops. Sequencing of the cyp51A gene and its promoter region indicated polymorphism, mainly with the presence of TR46/Y121F/T289A (n = 8), TR34/L98H, and TR53. Eight resistant isolates exhibited cyp51A wild type without alterations in the promoter region. Our study showed evidence of dissemination of azole-resistant A. fumigatus, with high genetic diversity, in vegetable crops in Colombia. These data underline the need to determine the prevalence of azole resistance in A. fumigatus in clinical and environmental settings for other regions of Colombia as well as Latin America.