Status of the crystallography beamlines at PETRA III
Status of the crystallography beamlines at PETRA III
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DOI：
10.1140/epjp/i2016-16056-0
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发表时间：
2016-03-14
影响因子：
3.4
通讯作者：
Meents, Alke
中科院分区：
文献类型：
--
作者：
Burkhardt, Anja;Pakendorf, Tim;Meents, Alke
自 2013 年以来，位于汉堡的第三代同步加速器 PETRA III 为用户提供了三种用于大分子晶体学的光束线：P11、P13 和 P14，后两者由 EMBL 运营。 Beamline P11 由 DESY 操作并配备 Pilatus 6M 探测器。加上 PETRA III 非常明亮的 X 射线源提供的 ph/s 光子通量，通常可以在不到 2 分钟的时间内收集完整的数据集。 P11 提供最先进的微聚焦功能，光束尺寸低至 μ m(2)，这使得光束线非常适合微晶体研究和系列晶体学实验。自动样品更换器可在 20 秒内快速更换样品，从而实现高通量晶体学和快速晶体筛选。对于样品制备，S2 生物安全实验室位于光束线附近。
Since 2013, three beamlines for macromolecular crystallography are available to users at the third-generation synchrotron PETRA III in Hamburg: P11, P13 and P14, the latter two operated by EMBL. Beamline P11 is operated by DESY and is equipped with a Pilatus 6M detector. Together with the photon flux of ph/s provided by the very brilliant X-ray source of PETRA III, a full data set can be typically collected in less than 2min. P11 provides state-of-the-art microfocusing capabilities with beam sizes down to mu m(2), which makes the beamline ideally suited for investigation of microcrystals and serial crystallography experiments. An automatic sample changer allows fast sample exchange in less than 20s, which enables high-throughput crystallography and fast crystal screening. For sample preparation, an S2 biosafety laboratory is available in close proximity to the beamline.