Osteoblasts Proliferation and Differentiation Stimulating Activities of the Main Components of Epimedii folium.
Osteoblasts Proliferation and Differentiation Stimulating Activities of the Main Components of Epimedii folium.
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淫羊藿主要成分对成骨细胞增殖分化的促进作用
DOI：
10.4103/0973-1296.197654
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发表时间：
2017-01
影响因子：
0.7
通讯作者：
Huang G
中科院分区：
文献类型：
--
作者：
Liu M;Xu H;Ma Y;Cheng J;Hua Z;Huang G
背景：骨质疏松症是一种导致骨折风险增加的骨骼疾病。中国使用淫羊藿叶治疗骨折和关节疾病已有几千年的历史。方法：本研究旨在筛选淫羊藿叶中可能具有促进成骨细胞增殖和分化的活性成分。建立了淫羊藿叶中主要成分的高效液相色谱分析方法。采用四甲基偶氮唑盐比色法和碱性磷酸酶比色法检测成骨细胞的增殖和分化活性。以黄酮类化合物巴瓦钦为阳性对照。结果：通过与相应标准品的保留时间比较，共鉴定出8个化合物。淫羊藿苷I和淫羊藿苷II显著促进细胞增殖和成骨细胞分化。所有这些化合物都在其分子骨架中发现了具有特征的类黄酮类结构。结论：这些结果导致了一种假说，即黄酮单糖苷结构可能是展示该活性的关键。这些化合物与小鼠原代颅骨成骨细胞中黄酮单糖苷结构的构效关系有待进一步研究。使用的缩写：高效液相色谱法，四甲基偶氮唑溴化铵，碱性磷酸酶
Background: Osteoporosis is a disease of bones that leads to an increased risk of fracture. Epimedii Folium is commonly used for treating bone fractures and joint diseases for thousands of years in China. Methods: This study was aimed to screen active components, which might have the potency to stimulate osteoblasts proliferation and differentiation in Epimedii Folium. An HPLC method was established to analyze the main components in Epimedii Folium. The MTT and ALP methods were utilized for the assay of osteoblasts proliferation and differentiation activity. Bavachin, a flavonoid compound was treated as the positive control. Results: Totally eight compounds have been identified by comparing their retention time with correspondent standard substances. Icariside I and icariside II significantly stimulated cell proliferation and osteoblasts differentiation. All these compounds were found with a characterized flavonoid structure in each of their molecule backbones. Conclusion: These results lead to a hypothesis that flavonoid monoglycoside structure might be crucial to exhibit the activity. The structure–effect relationship of these compounds with flavonoid monoglycoside structure in mouse primary calvarial osteoblasts needs to be explored in further research. Abbreviations used: HPLC: High performance liquid chromatography, MTT: Methylthiazolyldiphenyl - tetrazolium bromide, ALP: Alkaline phosphatase