Characterization of new fluorescent labels for ultra-high resolution microscopy
Characterization of new fluorescent labels for ultra-high resolution microscopy
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DOI：
10.1039/b810991p
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发表时间：
2008-01-01
影响因子：
3.1
通讯作者：
Widengren, Jerker
中科院分区：
文献类型：
--
作者：
Chmyrov, Andriy;Arden-Jacob, Jutta;Widengren, Jerker
作为实现超高光学分辨率的一种手段，光诱导染料切换到暗的、长寿命的状态，如三重态，最近得到了越来越多的关注。此外，这些长寿命状态通常对环境高度敏感，因此它们的光动力学可以提供额外的独立的基于荧光的信息。然而，尽管为光诱导开关提供了一种有用的机制，但三线态经常作为光漂白的前驱状态出现，这可能会限制其用途。在这项工作中，一组罗丹明和派罗宁染料，通过取代重原子和氮在染料的中心黄原烯单元内或附近，研究了它们的三重态动力学和光稳定性，在与超高分辨率显微镜相关的条件下。在所研究的染料中，特别是具有硫原子取代中心氧原子的罗丹明和派罗宁染料，被发现满足超高分辨率显微镜的要求，结合了显著的三重态产量和合理的光稳定性。
Photo-induced switching of dyes into dark, long-lived states, such as a triplet state, has recently gained increasing interest, as a means to achieve ultra-high optical resolution. Additionally, these long lived states are often highly environment-sensitive and their photodynamics can thus offer additional independent fluorescence-based information. However, although providing a useful mechanism for photo-induced switching, the triplet state often appears as a precursor state for photobleaching, which potentially can limit its usefulness. In this work, a set of rhodamine and pyronin dyes, modified by substitution of heavy atoms and nitrogen within or close to the central xanthene unit of the dyes, were investigated with respect to their triplet state dynamics and photostabilities, under conditions relevant for ultra-high resolution microscopy. Out of the dyes investigated, in particular the rhodamine and pyronin dyes with a sulfur atom replacing the central oxygen atom in the xanthene unit were found to meet the requirements for ultra-high resolution microscopy, combining a prominent triplet state yield with reasonable photostability.