A nanocatalyst-based assay for proteins:: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels
A nanocatalyst-based assay for proteins:: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels
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DOI：
10.1021/ja0672167
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发表时间：
2006-12-20
影响因子：
15
通讯作者：
Yang, Haesik
中科院分区：
文献类型：
--
作者：
Das, Jagotamoy;Aziz, Md. Abdul;Yang, Haesik
该通信报告了一种基于纳米催化剂的蛋白质电化学分析。超灵敏的检测已实现了信号放大与噪声抑制相结合：信号被放大的催化还原对硝基苯酚顶部-氨基苯酚的金纳米催化剂标记物和化学还原的对苯醌亚胺顶部-氨基苯酚的NaBH 4;噪声降低采用氧化铟锡电极修饰的二茂铁系树枝状大分子和亲水性免疫传感层。
This communication reports a nanocatalyst-based electrochemical assay for proteins. Ultrasensitive detection has been achieved by signal amplification combined with noise reduction: the signal is amplified both by the catalytic reduction ofp-nitrophenol top-aminophenol by gold-nanocatalyst labels and by the chemical reduction ofp-quinone imine top-aminophenol by NaBH4; the noise is reduced by employing an indium tin oxide electrode modified with a ferrocenyl-tethered dendrimer and a hydrophilic immunosensing layer.