Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development.
Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development.
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由于原肠胚发育受损而缺乏 Trim59 的小鼠的胚胎致死率
DOI：
10.1038/s41419-018-0370-y
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发表时间：
2018-02-21
影响因子：
9
通讯作者：
Yang R
中科院分区：
文献类型：
--
作者：
Su X;Wu C;Ye X;Zeng M;Zhang Z;Che Y;Zhang Y;Liu L;Lin Y;Yang R
TRIM家族成员参与了多种生物学过程，如分化和发育。我们发现Trim59在早期胚胎从囊胚期到原肠胚的发育中起着关键作用。Trim 59-/-胚胎从胚天（E）8.5开始就存在发育延迟和空卵黄囊。在E9.5以后没有观察到有活力的Trim59 −/−胚胎。Trim59缺乏影响原肠形成初期的初级胚层形成。在E6.5和E7.5时，Trim59 −/−胚胎中初级胚层形成相关基因（包括Brachyury、lefty2、Cer1、Otx2、Wnt3和BMP 4）的表达降低。纯合子突变体胚胎上胚层收缩，中胚层缺失。Trim59可以与肌动蛋白和肌球蛋白相关蛋白相互作用。它的缺乏会干扰内细胞团分化过程中的F-肌动蛋白聚合。Trim59介导的F-肌动蛋白聚合是通过WASH K63连接的泛素化。因此，Trim59可能通过调节F-actin组装而成为早期胚胎从囊胚期到原肠胚发育的关键调节因子。
TRIM family members have been implicated in a variety of biological processes such as differentiation and development. We here found that Trim59 plays a critical role in early embryo development from blastocyst stage to gastrula. There existed delayed development and empty yolk sacs from embryonic day (E) 8.5 inTrim59−/−embryos. No viableTrim59−/−embryos were observed beyond E9.5. Trim59 deficiency affected primary germ layer formation at the beginning of gastrulation. At E6.5 and E7.5, the expression of primary germ layer formation-associated genes includingBrachyury,lefty2,Cer1,Otx2,Wnt3,andBMP4was reduced inTrim59−/−embryos. Homozygous mutant embryonic epiblasts were contracted and the mesoderm was absent. Trim59 could interact with actin- and myosin-associated proteins. Its deficiency disturbed F-actin polymerization during inner cell mass differentiation. Trim59-mediated polymerization of F-actin was via WASH K63-linked ubiquitination. Thus, Trim59 may be a critical regulator for early embryo development from blastocyst stage to gastrula through modulating F-actin assembly.
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DOI：
10.1038/nrm2867
发表时间：
2010-04
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
16.2
作者：
Lanier, LM;Gates, MA;Gertler, FB
通讯作者：
Gertler, FB
影响因子：
2.7
作者：
Bassham, S;Postlethwait, J
通讯作者：
Postlethwait, J
影响因子：
2.8
作者：
Gaivao, Maria M. F.;Rambags, Bjorn P. B.;Stout, Tom A. E.
通讯作者：
Stout, Tom A. E.
影响因子：
4.6
作者：
Blitz, IL;Cho, KWY;Chang, CB
通讯作者：
Chang, CB