Development of a cerebrospinal fluid lateral reservoir model in rhesus monkeys (Macaca mulatta).
Development of a cerebrospinal fluid lateral reservoir model in rhesus monkeys (Macaca mulatta).
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发表时间：
2015-02
影响因子：
0.8
通讯作者：
Cynthia Lester McCully;J. Bacher;R. MacAllister;E. Steffen-Smith;K. Saleem;Marvin L. Thomas;Rafael Cruz;K. Warren
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作者：
Cynthia Lester McCully;J. Bacher;R. MacAllister;E. Steffen-Smith;K. Saleem;Marvin L. Thomas;Rafael Cruz;K. Warren
非人灵长类动物（NHP）脑脊液（CSF）的快速，连续和人道的收集是许多研究的基本要素，目前通过两种不同的模型完成。CSF储液器模型（FR）将第四脑室中的导管与柔性硅橡胶储液器结合，以允许循环CSF流动。CSF侧孔模型（LP）由侧脑室导管和IV孔组成，可提供CSF静态通路和样本采集体积限制。FR模型与密集、延长的恢复和频繁的术后脑积水和非通畅性相关，而LP模型与更容易恢复相关。为了最大限度地发挥两种系统的优势，我们开发了CSF侧储液囊模型（LR），该模型结合了之前2种模型的有益特征，但通过使用储液囊循环CSF流并结合侧脑室中的导管放置来避免其局限性。本研究中使用了9只成年雄性恒河猴。术前行MRI检查以确定侧脑室的坐标和脉络丛的位置。确定坐标以避开CP和主要血管。根据MRI验证，预定坐标100%准确。LR系统在67%的病例中成功发挥功能221天，44%的病例在术后426至510天保持功能。与已建立的模型相比，我们的LR模型明显减少了术后并发症和恢复时间。LR模型的开发在恒河猴中是成功的，并且是从非人灵长类动物收集CSF的FR和LP方法的有用替代方法。
Rapid, serial, and humane collection of cerebrospinal fluid (CSF) in nonhuman primates (NHP) is an essential element of numerous research studies and is currently accomplished via two different models. The CSF reservoir model (FR) combines a catheter in the 4th ventricle with a flexible silastic reservoir to permit circulating CSF flow. The CSF lateral port model (LP) consists of a lateral ventricular catheter and an IV port that provides static access to CSF and volume restrictions on sample collection. The FR model is associated with an intensive, prolonged recovery and frequent postsurgical hydrocephalus and nonpatency, whereas the LP model is associated with an easier recovery. To maximize the advantages of both systems, we developed the CSF lateral reservoir model (LR), which combines the beneficial features of the 2 previous models but avoids their limitations by using a reservoir for circulating CSF flow combined with catheter placement in the lateral ventricle. Nine adult male rhesus monkeys were utilized in this study. Pre-surgical MRI was performed to determine the coordinates of the lateral ventricle and location of choroid plexus (CP). The coordinates were determined to avoid the CP and major blood vessels. The predetermined coordinates were 100% accurate, according to MRI validation. The LR system functioned successfully in 67% of cases for 221 d, and 44% remain functional at 426 to 510 d postoperatively. Compared with established models, our LR model markedly reduced postoperative complications and recovery time. Development of the LR model was successful in rhesus macaques and is a useful alternative to the FR and LP methods of CSF collection from nonhuman primates.