Anti-inflammatory effects of CoQ10 and colorless carotenoids.
Anti-inflammatory effects of CoQ10 and colorless carotenoids.
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DOI：
10.1111/j.1473-2165.2006.00220.x
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发表时间：
2006-03-01
影响因子：
2.3
通讯作者：
Kern, Dale
中科院分区：
文献类型：
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作者：
Fuller, Bryan;Smith, Dustin;Kern, Dale
背景：辅酶Q10（泛醌，辅酶Q10）和类胡萝卜素是许多护肤品中常用的抗氧化剂，用于保护皮肤免受自由基损伤。目的：评价辅酶q10和无色类胡萝卜素对紫外线照射下人真皮成纤维细胞炎症介质生成的影响，并探讨这两种抗氧化剂可能的协同作用。方法：将正常的人真皮成纤维细胞培养物暴露于50 mJ的UVR或IL-1中，然后与不同浓度的辅酶q10、无色类胡萝卜素、植物烯和植物荧光素或这些抗氧化剂的组合一起培养。培养24小时后，收集细胞和废培养基，用酶联免疫吸附法检测前列腺素E2 （PGE-2）、白细胞介素6 （IL-6）和基质金属蛋白酶1 （MMP-1）。此外，还确定了类胡萝卜素保护辅酶q10免受活性氧（ROS）（高氯酸盐）氧化的能力。结果：人成纤维细胞通过增加各种炎症介质（包括PGE-2、IL-1和IL-6）和蛋白酶（如胶原酶（MMP-1））的产生来响应UVR或IL-1。用10微米CoQ10处理成纤维细胞抑制UVR-或il -1诱导的PGE-2、IL-6和MMP-1的增加。类胡萝卜素和辅酶q10的结合对这三种炎症介质产生了增强的抑制作用。此外，无色的类胡萝卜素，植物烯和植物荧光素，保护辅酶q10不被活性氧，次氯酸盐降解。结论：CoQ10能够抑制UVR-或il -1诱导的真皮成纤维细胞炎症反应。此外，该化合物可以阻断基质侵蚀酶MMP-1的UVR诱导。最后，类胡萝卜素和辅酶q10的结合可以增强对炎症的抑制。结果表明，类胡萝卜素和辅酶q10在局部护肤产品中的组合可能提供增强的保护，防止日晒引起的炎症和过早衰老。
BACKGROUND: CoQ10 (ubiquinone, coenzyme Q10) and carotenoids are popular antioxidants used in many skin care products to protect the skin from free radical damage.AIM: To evaluate the effects of CoQ10 and colorless carotenoids on the production of inflammatory mediators in human dermal fibroblasts treated with UV radiation (UVR) and to investigate the possible synergistic effects of these two antioxidants.METHODS: Normal human dermal fibroblast cell cultures were exposed to either 50 mJ of UVR or to IL-1 and then incubated with various concentrations of either CoQ10, the colorless carotenoids, phytoene and phytofluene, or to combinations of these antioxidants. After 24 h in culture, cells and spent medium were harvested and assayed by enzyme-linked immunosorbent assay for prostaglandin E2 (PGE-2), interleukin 6 (IL-6), and matrix metalloproteinase 1 (MMP-1). In addition, the ability of the carotenoids to protect CoQ10 from oxidation by the reactive oxygen species (ROS), hyperchlorite, was also determined.RESULTS: Human fibroblasts respond to UVR or to IL-1 by increasing the production of various inflammatory mediators including PGE-2, IL-1, and IL-6 and proteases such as collagenase (MMP-1). Treatment of fibroblasts with 10 microm of CoQ10 suppressed the UVR- or IL-1-induced increase in PGE-2, IL-6, and MMP-1. The combination of carotenoids and CoQ10 produced an enhanced inhibition of these three inflammatory mediators. Furthermore, the colorless carotenoids, phytoene and phytofluene, protected CoQ10 from degradation by the ROS, hypochlorite.CONCLUSION: CoQ10 is able to suppress the UVR- or IL-1-induced inflammatory response in dermal fibroblasts. Furthermore, this compound can block the UVR induction of the matrix-eroding enzyme, MMP-1. Finally, the combination of carotenoids plus CoQ10 results in enhanced suppression of inflammation. The results suggest that the combination of carotenoids and CoQ10 in topical skin care products may provide enhanced protection from inflammation and premature aging caused by sun exposure.