Mechanical touch responses of Arabidopsis TCH1-3 mutant roots on inclined hard-agar surface**
Mechanical touch responses of Arabidopsis TCH1-3 mutant roots on inclined hard-agar surface**
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DOI：
10.1515/intag-2015-0065
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发表时间：
2016
影响因子：
2.2
通讯作者：
Guodong Zha;Bochu Wang;Junyu Liu;Jie Yan;Liqing Zhu;Xingyan Yang
中科院分区：
文献类型：
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作者：
Guodong Zha;Bochu Wang;Junyu Liu;Jie Yan;Liqing Zhu;Xingyan Yang
摘要 重力引起的机械触摸刺激会影响植物根系结构。植物根系的机械触摸反应是植物根系生长发育的一个重要方面。先前的研究报道拟南芥TCH1-3基因参与机械相关事件，然而TCH1-3基因在拟南芥根部机械反应中的生理功能仍不清楚。在本研究中，我们应用倾斜硬琼脂平板方法产生机械触摸刺激，并提供了改变机械环境可能影响根系生长的证据。此外，在倾斜琼脂表面上研究了 tch1-3 拟南芥突变体，以探索 TCH1-3 基因对拟南芥根机械反应的功能。结果表明，两个tch2突变体cml24-2和cml24-4表现出根长显着缩短、偏斜和侧根密度降低。此外，tch3（cml12-2）的初生根长度和侧根密度在倾斜琼脂表面上显着降低。本研究表明tch2和tch3突变体对机械触摸刺激高度敏感，TCH2（CML24-2和CML24-4）和TCH3（CML12-2）基因可能参与拟南芥根部的机械触摸反应。
Abstract The gravity-induced mechanical touch stimulus can affect plant root architecture. Mechanical touch responses of plant roots are an important aspect of plant root growth and development. Previous studies have reported that Arabidopsis TCH1-3 genes are involved in mechano-related events, how-ever, the physiological functions of TCH1-3 genes in Arabidopsis root mechanoresponses remain unclear. In the present study, we applied an inclined hard agar plate method to produce mechanical touch stimulus, and provided evidence that altered mechanical environment could influence root growth. Furthermore, tch1-3 Arabidopsis mutants were investigated on inclined agar surfaces to explore the functions of TCH1-3 genes on Arabidopsis root mechanoresponses. The results showed that two tch2 mutants, cml24-2 and cml24-4, exhibited significantly reduced root length, biased skewing, and decreased density of lateral root. In addition, primary root length and density of lateral root of tch3 (cml12-2) was significantly decreased on inclined agar surfaces. This study indicates that the tch2 and tch3 mutants are hypersensitive to mechanical touch stimulus, and TCH2 (CML24-2 and CML24-4) and TCH3 (CML12-2) genes may participate in the mechanical touch response of Arabidopsis roots.