Phylogenetic analysis of Metarhizium spp. isolated from soil in Japan
Phylogenetic analysis of Metarhizium spp. isolated from soil in Japan
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DOI：
10.1007/s13355-011-0045-y
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发表时间：
2011-08-01
影响因子：
1.3
通讯作者：
Shimizu, Susumu
中科院分区：
文献类型：
--
作者：
Nishi, Oumi;Hasegawa, Keiichi;Shimizu, Susumu
通过对145个绿僵菌菌株的内转录间隔区（ITS）和EF-1 α 5'端序列的分析，发现绿僵菌EF-1 α 5'端序列与其它菌株的ITS序列一致。分离自日本土壤，使用选择性琼脂培养基，鉴定了8个种。ITS序列分析将分离物分为3个分支。其中两个被鉴定为M。黄绿色变种pemphigi和M. lepidiotae。EF-1 α序列分析鉴定了其他进化枝为6个种：M. anisopliae、绿僵菌M. brunneum、褐毛叶蝉M. guizhouense、M. majus，M. pingshaense和M. robertisii。研究了M.黄绿色变种pemphigi仅限于森林或林地土壤，M. guizhouense和M. majus与基于EF-1 α 5'端序列的同源性不一致。
As a result of analyzing the internal transcribed spacer (ITS) and 5' end of the EF-1 alpha sequence of 145 isolates of Metarhizium spp. isolated from soil in Japan using selective agar medium, eight species were identified. ITS sequence analysis divided the isolates into three clades. Two were identified as M. flavoviride var. pemphigi and M. lepidiotae, respectively. EF-1 alpha sequence analysis identified the other clades as six species: M. anisopliae, M. brunneum, M. guizhouense, M. majus, M. pingshaense and M. robertisii. The distribution of M. flavoviride var. pemphigi was restricted to forest or wood soil, and conidial sizes of M. guizhouense and M. majus were incongruent with the phylogeny based on the sequence of the 5' end of EF-1 alpha.