The encapsulin from Thermotoga maritima is a flavoprotein with a symmetry matched ferritin-like cargo protein.
The encapsulin from Thermotoga maritima is a flavoprotein with a symmetry matched ferritin-like cargo protein.
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DOI：
10.1038/s41598-021-01932-w
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发表时间：
2021-11-23
影响因子：
4.6
通讯作者：
Savage DF
中科院分区：
文献类型：
--
作者：
LaFrance BJ;Cassidy-Amstutz C;Nichols RJ;Oltrogge LM;Nogales E;Savage DF
细菌纳米室，也称为封装蛋白，是在细菌和古细菌中发现的一类新兴的基于蛋白质的“细胞器”。封装蛋白是病毒样二十面体颗粒，包含围绕特定货物酶的约 25-50 nm 外壳。隔室化被认为可以创造一个独特的化学环境，以促进催化并隔离有毒中间体。有关纳米室结构-功能的许多问题仍未得到解答，包括壳对称性如何决定货物装载以及壳在多大程度上促进酶活性。在这里，我们使用已知封装氧化还原活性铁蛋白样蛋白的海栖热袍菌纳米室模型来探索这些问题。生化分析显示，封装蛋白壳在壳粒亚基之间的界面处具有黄素结合位点，表明封装蛋白壳可能在封装货物的功能中发挥直接和积极的作用。此外，我们使用冷冻电镜证明货物蛋白使用一种对称匹配形式来促进封装并定义化学计量。就海栖热袍菌封装蛋白而言，具有五重对称性的十聚体货物蛋白优先结合二十面体壳的五聚轴。总而言之，这些观察结果表明，外壳不仅仅是一个被动屏障，它还在货物酶的结构和功能中发挥着重要作用。
Bacterial nanocompartments, also known as encapsulins, are an emerging class of protein-based ‘organelles’ found in bacteria and archaea. Encapsulins are virus-like icosahedral particles comprising a ~ 25–50 nm shell surrounding a specific cargo enzyme. Compartmentalization is thought to create a unique chemical environment to facilitate catalysis and isolate toxic intermediates. Many questions regarding nanocompartment structure–function remain unanswered, including how shell symmetry dictates cargo loading and to what extent the shell facilitates enzymatic activity. Here, we explore these questions using the model Thermotoga maritima nanocompartment known to encapsulate a redox-active ferritin-like protein. Biochemical analysis revealed the encapsulin shell to possess a flavin binding site located at the interface between capsomere subunits, suggesting the shell may play a direct and active role in the function of the encapsulated cargo. Furthermore, we used cryo-EM to show that cargo proteins use a form of symmetry-matching to facilitate encapsulation and define stoichiometry. In the case of the Thermotoga maritima encapsulin, the decameric cargo protein with fivefold symmetry preferentially binds to the pentameric-axis of the icosahedral shell. Taken together, these observations suggest the shell is not simply a passive barrier—it also plays a significant role in the structure and function of the cargo enzyme.
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影响因子：
16.8
作者：
Sutter, Markus;Boehringer, Daniel;Ban, Nenad
通讯作者：
Ban, Nenad
影响因子：
7.7
作者：
He, Didi;Hughes, Sam;Marles-Wright, Jon
通讯作者：
Marles-Wright, Jon
影响因子：
48
作者：
Zheng SQ;Palovcak E;Armache JP;Verba KA;Cheng Y;Agard DA
通讯作者：
Agard DA
影响因子：
5.4
作者：
Rahmanpour, Rahman;Bugg, Timothy D. H.
通讯作者：
Bugg, Timothy D. H.
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH