Identification and characterization of human BIC, a gene on chromosome 21 that encodes a noncoding RNA
Identification and characterization of human BIC, a gene on chromosome 21 that encodes a noncoding RNA
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DOI：
10.1016/s0378-1119(01)00612-6
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发表时间：
2001-08-22
期刊：
影响因子：
3.5
通讯作者：
Tam, W
中科院分区：
文献类型：
--
作者：
Tam, W
BIC最初被鉴定为一个在禽白血病病毒诱导的B细胞淋巴瘤中，因启动子插入一个常见的逆转录病毒整合位点而转录激活的基因（Tam等人，《分子细胞生物学》17卷（1997年）1490页）。该基因的人类同源物被克隆并进行了特征分析。它在位于21号染色体q21区域的13kb范围内由三个外显子组成。与禽同源物相似，人类BIC缺乏一个长的开放阅读框（ORF）。通过Northern分析在脾脏和胸腺中检测到BIC的最高表达水平。此外，还鉴定出了BIC的小鼠同源物。对来自人类、小鼠和鸡的BIC cDNA进行比较，发现在138个核苷酸上有78%的一致性。然而，这些cDNA中存在的多个短ORF之间没有同源性。通过计算机分析预测，序列同源区域形成一个不完美的RNA双链体，在这三个物种中结构相似。基于缺乏保守的ORF以及RNA二级结构的进化保守性，我们推测BIC作为一种非编码RNA发挥作用。（C）2001年，爱思唯尔科学出版公司。保留所有权利。
BIC was originally identified as a gene transcriptionally activated by promoter insertion at a common retroviral integration site in B cell lymphomas induced by avian leukosis virus (Tam et al., Mol. Cell. Biol. 17 (1997) 1490). The human homolog of this gene was cloned and characterized. It consists of three exons within a 13 kb region located in chromosome 21q21. Similar to the avian homolog, the human BIC lacks a long open reading frame (ORF). Highest levels of BIC expression are detected in the spleen and thymus by Northern analysis. In addition, the mouse homolog of BIC was identified. Comparison of BIC cDNAs from human, mouse and chicken reveals 78% identity over 138 nucleotides. However, there is no homology among the multiple short ORFs present in these cDNAs. The region of sequence homolog gy is predicted by computer analysis to form an imperfect RNA duplex, which is structurally similar among the three species. Based on the lack of a conserved ORF and the evolutionary conservation of RNA secondary structure, we presume that BIC functions as a noncoding RNA. (C) 2001 Elsevier Science B.V. All rights reserved.