Elevated endogenous GABA level correlates with decreased fMRI signals in the rat brain during acute inhibition of GABA transaminase
Elevated endogenous GABA level correlates with decreased fMRI signals in the rat brain during acute inhibition of GABA transaminase
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DOI：
10.1002/jnr.20364
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发表时间：
2005-02-01
影响因子：
4.2
通讯作者：
Shen, J
中科院分区：
文献类型：
--
作者：
Chen, ZG;Silva, AC;Shen, J
GABA（γ-氨基丁酸）转氨酶的高度特异性抑制剂氨己烯酸和gabaculine引起脑中内源性GABA水平的显著升高。在α-氯醛糖麻醉的大鼠脑中，使用体内选择性Homoclonal偏振转移光谱定量测量急性GABA转氨酶抑制后GABA浓度的时间过程。功能磁共振成像（fMRI）中的血氧水平依赖性（BOLD）效应被认为是通过相关谷氨酸转运的代谢需求与神经元激活紧密耦合的。与内源性GABA水平的上升后，氨己烯酸或gabaculine治疗，BOLD加权功能磁共振成像信号的强度在大鼠体感皮层在前爪刺激被发现显着降低。这些结果与先前的发现一致，即抑制GABA转氨酶导致GABA释放增加和增强GABA能抑制。(C)2004 Wiley-Liss，Inc.
Vigabatrin and gabaculine, both highly specific inhibitors of GABA (gamma-aminobutyric acid) transaminase, cause significant elevation of endogenous GABA levels in brain. The time course of GABA concentration after acute GABA transaminase inhibition was measured quantitatively in the alpha-chloralose-anesthetized rat brain using in vivo selective homonuclear polarization transfer spectroscopy. The blood oxygenation level-dependent (BOLD) effect in functional magnetic resonance imaging (fMRI) has been considered to be coupled tightly to neuronal activation via the metabolic demand of associated glutamate transport. Correlated with the rise in endogenous GABA level after vigabatrin or gabaculine treatment, the intensity of BOLD-weighted fMRI signals in rat somatosensory cortex during forepaw stimulation was found to be reduced significantly. These results are consistent with previous findings that inhibition of GABA transaminase leads to augmented GABA release and potentiation of GABAergic inhibition. (C) 2004 Wiley-Liss, Inc.