Three-dimensional in vivo fluorescence diffuse optical tomography of breast cancer in humans
Three-dimensional in vivo fluorescence diffuse optical tomography of breast cancer in humans
复制标题
DOI：
10.1364/oe.15.006696
复制
发表时间：
2007-05-28
期刊：
影响因子：
3.8
通讯作者：
Yodh, Arjun G.
中科院分区：
文献类型：
--
作者：
Corlu, Alper;Choe, Regine;Yodh, Arjun G.
我们提出了三维（3D）在体内的人类乳腺癌的荧光扩散光学断层扫描（FDOT）的基础上的图像。据我们所知，这项工作是第一次报告的三维荧光断层扫描的人乳腺癌在体内。在我们的方案中，荧光团吲哚菁绿色（ICG）静脉注射。荧光激发和检测是在软压缩，平行平面，使用786 nm的激光源和光谱过滤CCD检测传输几何完成。体模和体内研究证实，信号是由于ICG荧光，而不是组织自体荧光和激发光泄漏。乳腺肿瘤的荧光图像与MRI和基于内源性对比度的DOT图像具有良好的一致性。基于ICG荧光的肿瘤与正常组织对比度比基于血红蛋白和散射参数的对比度高2至4倍。总之，测量表明乳腺癌的FDOT是可行的和有前途的。(C)2007年，美国光学学会。
We present three-dimensional (3D) in vivo images of human breast cancer based on fluorescence diffuse optical tomography (FDOT). To our knowledge, this work represents the first reported 3D fluorescence tomography of human breast cancer in vivo. In our protocol, the fluorophore Indocyanine Green (ICG) is injected intravenously. Fluorescence excitation and detection are accomplished in the soft-compression, parallel-plane, transmission geometry using laser sources at 786 nm and spectrally filtered CCD detection. Phantom and in vivo studies confirm the signals are due to ICG fluorescence, rather than tissue autofluorescence and excitation light leakage. Fluorescence images of breast tumors were in good agreement with those of MRI, and with DOT based on endogenous contrast. Tumor-to-normal tissue contrast based on ICG fluorescence was two-to-four-fold higher than contrast based on hemoglobin and scattering parameters. In total the measurements demonstrate that FDOT of breast cancer is feasible and promising. (C) 2007 Optical Society of America.