Genome-Wide Analysis of Odorant-Binding Proteins in Papilio xuthus with Focus on the Perception of Two PxutGOBPs to Host Odorants and Insecticides.
Genome-Wide Analysis of Odorant-Binding Proteins in Papilio xuthus with Focus on the Perception of Two PxutGOBPs to Host Odorants and Insecticides.
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DOI：
10.1021/acs.jafc.2c03396
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发表时间：
2022-08
影响因子：
6.1
通讯作者：
Ningna Yin;An-Jin Yang;Chun Wu;Hai-Yan Xiao;Yunshan Guo;Naiyong Liu
中科院分区：
文献类型：
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作者：
Ningna Yin;An-Jin Yang;Chun Wu;Hai-Yan Xiao;Yunshan Guo;Naiyong Liu
在这项研究中，我们用4个新基因和7个改进的序列注释了白凤蝶中的49个气味结合蛋白(OBP)。表达谱在触角或生殖组织中发现了大量的OBP。以两个天线增强型通用OBP(PxutGOBP1和PxutGOBP2)为靶标，采用反向化学生态策略筛选了三个关键化合物。其中，产卵刺激剂Vhenin-2与PxutGOBP1有很强的相互作用，解离常数(Ki)为10.34±0.07μM。分子模拟和定点突变表明His66、Thr73和Phe118在PxutGOBP1和Vicenin-2相互作用中具有重要作用。另外两种化合物，一种普通的花香β-紫罗兰酮和一种广泛使用的杀虫剂毒死蜱，对PxutGOBPs(Ki<；13μM)表现出高度的亲和力。此外，PxutGOBP1的两个突变His66Ala和Thr73Ala显著降低了与毒死蜱的结合。我们的研究为PxutGOBPs在气味感知中的可能作用提供了见解，并确定了PxutGOBP1与维菌素-2和毒死蜱的关键结合位点。
In this study, we annotated 49 odorant-binding proteins (OBPs) in Papilio xuthus, with four novel genes and seven improved sequences. Expression profiles identified numerous OBPs in antennae or reproductive tissues. Using two antenna-enriched general OBPs (PxutGOBP1 and PxutGOBP2) as targets, we screened three key compounds by a reverse chemical ecology strategy. Of these, an oviposition stimulant vicenin-2 could strongly interact with PxutGOBP1, representing a dissociation constant (Ki) value of 10.34 ± 0.07 μM. Molecular simulations and site-directed mutagenesis revealed the importance of His66, Thr73, and Phe118 between PxutGOBP1 and vicenin-2 interactions. Two other compounds, an ordinary floral scent β-ionone and a widely used insecticide chlorpyrifos, exhibited high affinities to PxutGOBPs (Ki < 13 μM). Furthermore, two mutations His66Ala and Thr73Ala of PxutGOBP1 significantly reduced the binding to chlorpyrifos. Our study provides insights into the putative roles of PxutGOBPs in odorant perception and identifies key binding sites of PxutGOBP1 to vicenin-2 and chlorpyrifos.