Practices of shake-flask culture and advances in monitoring CO2 and O2
Practices of shake-flask culture and advances in monitoring CO2 and O2
复制标题
摇瓶培养的实践以及 CO2 和 O2 监测的进展
DOI：
10.1007/s00253-018-8922-8
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发表时间：
2018
影响因子：
5
通讯作者：
Hideki Aoyagi
中科院分区：
文献类型：
--
作者：
Masato Takahashi;Hideki Aoyagi
自从震动文化被发明以来，大约已经过去了85年。从那时起，开发了各种监测设备来测量培养参数。在摇瓶培养中，细胞呼吸所消耗的二氧化碳和产生的二氧化碳是至关重要的，因为它们存在于培养液和摇瓶的顶空中。在摇瓶培养过程中，监测现场条件有助于分析o2和CO2的行为，它们根据亨利定律相互作用，并且比需要中断摇瓶的传统采样更方便。摇瓶培养的原位监测装置分为直接监测装置和最近开发的旁路监测装置。了解每种类型的特征及其对摇瓶培养的意外影响是很重要的，以便改进现有的设备和培养条件。旁路监测装置的技术发展是未来的迫切需要。了解传统摇瓶培养的机制也是必要的。现有的摇瓶培养方法可以扩展到下一代摇瓶培养中，通过根据摇瓶培养的预期目的，明智地选择监测设备，形成一种新的培养环境。建立和共享适合于摇瓶培养的各种监测设备和测量仪器的数据库，可以为扩大摇瓶培养细胞的应用提供宝贵的资源。
About 85 years have passed since the shaking culture was devised. Since then, various monitoring devices have been developed to measure culture parameters. O2consumed and CO2produced by the respiration of cells in shaking cultures are of paramount importance due to their presence in both the culture broth and headspace of shake flask. Monitoring in situ conditions during shake-flask culture is useful for analysing the behaviour of O2and CO2, which interact according to Henry’s law, and is more convenient than conventional sampling that requires interruption of shaking. In situ monitoring devices for shake-flask cultures are classified as direct or the recently developed bypass type. It is important to understand the characteristics of each type along with their unintended effect on shake-flask cultures, in order to improve the existing devices and culture conditions. Technical developments in the bypass monitoring devices are strongly desired in the future. It is also necessary to understand the mechanism underlying conventional shake-flask culture. The existing shaking culture methodology can be expanded into next-generation shake-flask cultures constituting a novel culture environment through a judicious selection of monitoring devices depending on the intended purpose of shake-flask culture. Construction and sharing the databases compatible with the various types of the monitoring devices and measurement instruments adapted for shaking culture can provide a valuable resource for broadening the application of cells with shake-flask culture.