Expression of chemokine receptors in human gastric cancer
Expression of chemokine receptors in human gastric cancer
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DOI：
10.1159/000085587
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发表时间：
2005-01-01
期刊：
影响因子：
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通讯作者：
Yang, HK
中科院分区：
文献类型：
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作者：
Kwak, MK;Hur, K;Yang, HK
趋化因子受体在肿瘤转移过程中的作用继续引起研究关注。在这里，我们评估了趋化因子受体CCR7和CXCR4在胃癌中的表达谱，以及它们作为预后标志物的潜在用途。采用逆转录聚合酶链式反应(RT-PCR)检测10株人胃癌细胞系和43例胃癌组织中CCR7和CXCR4的表达，免疫组织化学方法检测307例胃癌组织中CCR7和CXCR4的表达。同时结合这两种细胞因子受体的表达，对患者的临床病理特征和预后进行评估。CCR7和CXCR4在所有胃癌细胞系中均有表达，而在胃癌组织中的表达率分别为83.7%(36/43)和100%(43/43)。307例胃癌组织中CCR7和CXCR4的表达分别为22.5%(69/307)和36.5%(112/307)。免疫组织化学多因素分析显示，CCR7在分化型胃癌中的表达显著高于未分化癌(35.1vs.15.3%，p<；0.001)，在肠腺癌中的表达高于弥漫型癌(58.8vs.22.3%，p<；0.001)。然而，与以往的研究相比，CCR7和CXCR4的表达与淋巴结转移无关。此外，CCR7阳性的肿瘤患者的预后好于CCR7阴性的肿瘤患者，但CXCR4的表达与预后无相关性。综上所述，趋化因子受体CCR7和CXCR4分别在分化型和肠型胃癌中高表达。版权所有(C)2005 S.Karger AG，巴塞尔。
The roles of chemokine receptors in cancer metastatic processes continue to draw research attention. Here we evaluated the expression profiles of the chemokine receptors CCR7 and CXCR4 in gastric cancer, and their potential use as prognostic markers. The expressions of CCR7 and CXCR4 mRNA were analyzed by RT-PCR in 10 human gastric cancer cell lines and in 43 gastric cancer tissues, and in an additional 307 gastric cancer tissues by immunohistochemistry. Clinicopathological features and the prognoses of patients were also evaluated versus the expression of these two cytokine receptors. CCR7 and CXCR4 mRNA were found to be expressed in all gastric cancer cell lines, whereas their mRNA expression rates in gastric cancer tissues were 83.7% (36/43) and 100% (43/43), respectively. Immunohistochemical staining of the 307 gastric cancer tissues showed that the expression rates of CCR7 and CXCR4 were 22.5% (69/307) and 36.5% (112/307), respectively. Multivariate analysis of the immunohistochemistry results showed that the expression rate of CCR7 was significantly higher in differentiated than in undifferentiated gastric cancer types (35.1 vs. 15.3%, p < 0.001), and that CXCR4 was expressed at a higher rate in intestinal cancer than in diffuse-type cancer (58.8 vs. 22.3%, p < 0.001). However, in contrast to previous studies, the expressions of CCR7 or CXCR4 were not associated with lymph node metastasis. Moreover, the prognosis of patients with CCR7-positive tumors was better than that of patients with CCR7-negative tumors, but no such correlation was observed for CXCR4 expression. In conclusion, the expressions of the chemokine receptors CCR7 and CXCR4 were found to be high in differentiated and intestinal-type gastric cancers, respectively. Copyright (C) 2005 S. Karger AG, Basel.