Pretreatment with ChOH/Urea under mild conditions for the efficient deconstruction of poplar cell wall
Pretreatment with ChOH/Urea under mild conditions for the efficient deconstruction of poplar cell wall
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DOI：
10.1016/j.indcrop.2023.117316
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发表时间：
2023-11
影响因子：
5.9
通讯作者：
Li Li-Li;Xun Zhang;Ruimin Che;Maozhi Zhang;Feng Xu
中科院分区：
文献类型：
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作者：
Li Li-Li;Xun Zhang;Ruimin Che;Maozhi Zhang;Feng Xu
本研究采用由氢氧化胆碱（ChOH）和尿素（Ur）组成的硫酸二乙酯（DES）在温和条件（60 ℃）下对白杨进行预处理。预处理10 min的样品的72 h糖化效率显著提高（69.97%），这相当于比原料（29.89%）的糖产量增加2.4倍。这种显著的增强可能与白杨细胞壁中发生的拓扑化学和形态变化有关，包括木质素和半纤维素的去除，结晶度降低和微观结构破坏。值得注意的是，X-射线衍射分析表明，纤维素I经历了一个结晶转化过程中，从纤维素I纤维素II DES预处理。研究表明，在温和条件下，甲醇/尿素预处理能有效地解构白杨细胞壁，提高纤维素酶解效率。
In this study, DES consisting of Choline hydroxide (ChOH) and Urea (Ur) was used to pretreat poplar under mild conditions (60 ℃). The 72 h saccharification efficiency of the pretreated samples for 10 min was significantly improved (69.97 %), which amounted to a 2.4-fold increase in sugar yield over the raw material (29.89 %). The remarkable enhancement could be correlated to the topochemical and morphological changes occurring in poplar cell walls, including the removal of lignin and hemicelluloses, decreased crystallinity and microstructural disruption. Noteworthy, X-ray diffraction analysis showed that cellulose I underwent a crystallographic transformation from cellulose I to cellulose II during DES pretreatment. Our study demonstrated that ChOH/Ur pretreatment was quite effective in deconstructing poplar cell walls and increasing the enzymatic hydrolysis efficiency of cellulose under mild conditions.