Migration of glial cells differentiated from neurosphere-forming neural stem/progenitor cells depends on the stiffness of the chemically cross-linked collagen gel substrate
Migration of glial cells differentiated from neurosphere-forming neural stem/progenitor cells depends on the stiffness of the chemically cross-linked collagen gel substrate
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DOI：
10.1016/j.neulet.2013.09.012
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发表时间：
2013-10
影响因子：
2.5
通讯作者：
Hideki Mori;A. Takahashi;A. Horimoto;Masayuki Hara
中科院分区：
文献类型：
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作者：
Hideki Mori;A. Takahashi;A. Horimoto;Masayuki Hara
基质硬度影响细胞迁移和铺展。我们的研究表明，细胞粘附基质的刚度影响神经细胞的迁移模式。我们观察了从神经球形成神经干/祖细胞（NSPCs）分化的神经细胞在胶原凝胶上的迁移，胶原凝胶具有通过化学交联实现的不同程度的刚度。当以硬胶原凝胶（G′ = 5.5kPa，G″ = 0.2kPa）为基质时，胶质细胞和神经元细胞均广泛地扩散和迁移。而胶原软凝胶（G′ = 0.8kPa，G″ = 0.2kPa）则在一定范围内抑制了胶质细胞的迁移。软胶原凝胶上的胶质细胞中很少观察到丝状伪足。从NSPCs分化后，最近的神经细胞之间的细胞间距离的分析表明，胶质细胞更广泛地传播在硬胶原凝胶比软凝胶。免疫细胞化学分析显示迁移的细胞主要为胶质细胞，提示胶质细胞的迁移依赖于基质的硬度。
Substrate stiffness affects cell migration and spreading. Our study revealed that the stiffness of the cell-adhesive substrate affected the migration pattern of neural cells. We observed the migration of neural cells differentiated from neurosphere-forming neural stem/progenitor cells (NSPCs) on collagen gels with various degrees of stiffness achieved by chemical cross-linking. Both glial and neuronal cells broadly spread and migrated when stiff collagen gels (G′ = 5.5 kPa,G″ = 0.2 kPa) were used as the substrate. In contrast, the migration of glial cells was suppressed within the limited area on the soft collagen gels (G′ = 0.8 kPa,G″ = 0.2 kPa). Filopodia were rarely observed in glial cells on the soft collagen gels. Analysis of the intercellular distance between the closest neural cells after differentiation from NSPCs indicated that glial cells more broadly spread on the stiff collagen gels than on the soft gels. Immunocytochemical analysis showed that most of the migrated cells were glial cells, suggesting that migration of glial cells was dependent on the stiffness of substrate.