The physiologic role of CD19 cytoplasmic tyrosines
The physiologic role of CD19 cytoplasmic tyrosines
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DOI：
10.1016/s1074-7613(02)00426-0
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发表时间：
2002-10-01
期刊：
影响因子：
32.4
通讯作者：
Carter, RH
中科院分区：
文献类型：
--
作者：
Wang, Y;Brooks, SR;Carter, RH
在表达转基因CD19构建体的CD19敲除小鼠中检查了8种CD19酪氨酸的生理作用。CD 19 Y482和Y513对于正常B细胞生物学（包括131和边缘区B细胞的分化）以及对于T依赖性和非依赖性抗体应答是必需的。在CD 19 Y482和Y513突变的免疫小鼠中，早期生殖中心B细胞在表型和数量上表现正常，但生殖中心的成熟是有缺陷的。这与细胞周期进展迟缓有关。因此，Y482和Y513是体内CD 19所有功能所必需的。这些基因的突变降低了生发中心B细胞的增殖，为成熟失败提供了一种潜在的机制，从而消除了抗体应答。
The physiologic role of eight CD19 tyrosines was examined in CD19-knockout mice expressing transgenic CD19 constructs. CD19 Y482 and Y513 were essential for normal B cell biology, including differentiation of 131 and marginal zone B cells and for T-dependent and -independent antibody responses. In immunized mice with mutations in CD19 Y482 and Y513, early germinal center B cells appeared normal in phenotype and number, but maturation in the germinal center was defective. This was associated with retarded progression through the cell cycle. Thus, Y482 and Y513 are essential for all functions of CD19 in vivo. Mutation of these reduces proliferation in germinal center B cells, providing a potential mechanism for the failure of maturation, which abrogates antibody responses.