Correction to Compartment-Specific Labeling of Bacterial Periplasmic Proteins by Peroxidase-Mediated Biotinylation.
Correction to Compartment-Specific Labeling of Bacterial Periplasmic Proteins by Peroxidase-Mediated Biotinylation.
复制标题
通过过氧化物酶介导的生物素化校正细菌周质蛋白的区室特异性标记。
DOI：
10.1021/acsinfecdis.8b00143
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发表时间：
2018
影响因子：
5.3
通讯作者：
Seeliger,JessicaC
中科院分区：
文献类型：
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作者：
Ganapathy,UdayS;Bai,Lu;Wei,Linpeng;Eckartt,KathrynA;Lett,ClarissaM;Previti,MaryL;Carrico,IsaacS;Seeliger,JessicaC
在最初发表的版本中，方法章节引用了用于克隆APEX基因的模板质粒的错误AddGene参考编号，并提供了酪胺试剂的错误纯化条件。在“克隆”下，方法应为：“从pcDNA 3-mito-APEX（Addgene质粒#42607）亚克隆N-末端V5标记的APEX”。在酪胺叠氮化物和酪胺炔的合成下，纯化条件应为：“将残余物溶于乙腈中，并通过高压液相色谱法（HPLC）纯化（20 - 50%乙腈水溶液，含1%三氟乙酸，经85分钟，保留时间= 26分钟，35%乙腈）”。
In the original published version, the Methods section cited the incorrect AddGene reference number for the template plasmid used to clone the APEX gene and provided incorrect purification conditions for the tyramide reagents. Under Cloning, the methods should read:“N-terminal V5-tagged APEX was subcloned from pcDNA3-mito-APEX (Addgene plasmid# 42607).” Under Synthesis of Tyramide Azide and Tyramide Alkyne, the purification conditions should read:“The residue was dissolved in acetonitrile and purified by high-pressure liquid chromatography (HPLC)(20− 50% acetonitrile in water with 1% trifluoroacetic acid over 85 min, retention time= 26 min, 35% acetonitrile).”