Three‐dimensional reconstruction of tetraploid↔diploid chimaeric mouse blastocysts
Three‐dimensional reconstruction of tetraploid↔diploid chimaeric mouse blastocysts
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四倍体↔二倍体嵌合小鼠囊胚的三维重建
DOI：
10.1046/j.1469-7580.2000.19630341.x
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发表时间：
2000
影响因子：
2.4
通讯作者：
R. Baldock
中科院分区：
文献类型：
--
作者：
C. A. Everett;M. Stark;J. West;D. Davidson;R. Baldock
对四倍体↔二倍体(4N↔2n)小鼠嵌合体的研究表明，4N细胞对中期妊娠不同组织的贡献不同。这种模式早在E3.5d就已经在嵌合体中被报道，这表明4N细胞对壁状滋养外胚层的贡献增强(Everett&West，1996)。在本研究中，对4n↔2n和2n↔2n对照嵌合体囊胚进行了数字化和三维重建。3D图像显示，在两组嵌合体中，只有来自两个单独囊胚的贡献胚胎的细胞有限地混合。因此，两种细胞类型的分布模式取决于胚泡方向和两簇细胞之间边界之间的空间关系。观察到的4n↔2n和2n↔2n嵌合体的分布模式没有显著差异，在所有3个可识别的发育谱系中，每个嵌合体都显示了一些转基因阳性细胞的贡献。然而，值得注意的是，在所有的4N↔2n胚泡中，至少有一些4N细胞位于胚泡腔附近。这种一致的模式在2n↔2n嵌合体中并不明显。这项研究证明了三维重建在分析嵌合体小鼠囊胚中两个细胞群体的空间关系方面的价值。
Studies of tetraploid↔diploid (4n↔2n) mouse chimaeras have demonstrated unequal contributions of 4n cells to different tissues of the midgestation conceptus. Such a pattern has also been reported in chimaeras as early as E3.5d, which show an enhanced contribution of 4n cells to the mural trophectoderm (Everett & West, 1996). In this study, sectioned 4n↔2n and 2n↔2n control chimaeric blastocysts were digitised and reconstructed in 3 dimensions (3‐D). The 3‐D images revealed only limited mixing of cells from the 2 contributing embryos of individual blastocysts in both chimaera groups. Consequently, the distribution pattern of the 2 cell types was dependent on the spatial relationship between the orientation of the blastocyst and the boundary between the 2 clusters of cells. The distribution patterns observed were not strikingly different for 4n↔2n and 2n↔2n chimaeras, each showing some transgenic positive cell contribution in all 3 identifiable developmental lineages. It was notable, however, that in all 4n↔2n blastocysts at least some 4n cells were located adjacent to the blastocyst cavity. Such a consistent pattern was not evident in 2n↔2n chimaeras. This study has demonstrated the value of 3‐D reconstructions for the analysis of spatial relationships of 2 cell populations in chimaeric mouse blastocysts.
小鼠细胞核中染色体的组织：原位杂交分析。
影响因子：
4
作者：
Katsumata,M;Lo,CW
通讯作者：
Lo,CW
影响因子：
2.9
作者：
LO, CW;COULLING, M;KIRBY, C
通讯作者：
KIRBY, C
通过原位杂交定位组织切片中的低丰度 DNA 序列。
影响因子：
4
作者：
Lo,CW
通讯作者：
Lo,CW