Sensory system-predominant distribution of leukotriene A4 hydrolase and its colocalization with calretinin in the mouse nervous system
Sensory system-predominant distribution of leukotriene A4 hydrolase and its colocalization with calretinin in the mouse nervous system
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DOI：
10.1016/j.neuroscience.2006.04.012
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Hosokawa, M.
中科院分区：
文献类型：
--
作者：
Chiba, Y.;Shimada, A.;Hosokawa, M.
白三烯134是一种有效的脂质介质，已被鉴定为有效的促炎和免疫调节化合物。虽然有强有力的证据表明白三烯134在正常大脑中合成，但其在神经系统中的详细分布及其功能尚不清楚。为了深入了解白三烯1341可能的神经功能，我们检测了小鼠神经系统中白三烯A（4）水解酶的免疫组织化学分布，该酶催化白三烯B-4生物合成的最后和关键步骤。白三烯A4水解酶的免疫反应性分布广泛，且优先于感觉相关结构;即嗅上皮和犁鼻器中的神经元、嗅球、可能的无长突细胞、视网膜的神经节细胞层和内网状层中的三个带中的神经元、视神经和直到上级丘的束中的轴突，内毛细胞和外毛细胞以及耳蜗中的螺旋神经节细胞、前庭耳蜗神经束、三叉神经脊束和脊髓的第11层。双重免疫荧光染色表明，大多数白三烯A（4）-水解酶免疫阳性神经元共表达钙视网膜蛋白（神经元中的一种钙结合蛋白）。白三烯A4水解酶的普遍存在的分布与白三烯C-4合酶的分布形成鲜明对比[Shimada A，Satoh M，千叶Y，Saitoh Y，Kawamura N，Keino H，Hosokawa M，Shimizu T（2005）Highly selective localization of leukotriene C4 synthase in hypothalamic and extrahypothalamic vasopressin systems of mouse brain. Neuroscience 131：683-689]，其局限于下丘脑和下丘脑外的加压素能神经元。这些结果表明，白三烯134可能发挥一些神经调节功能，主要是在感觉神经系统，在音乐会与钙视网膜蛋白。(c)2006年IBRO。由Elsevier Ltd.出版。保留所有权利。
Leukotriene 134 is a potent lipid mediator, which has been identified as a potent proinflammatory and immunomodulatory compound. Although there has been robust evidence indicating that leukotriene 134 is synthesized in the normal brain, detailed distribution and its functions in the nervous system have been unclear. To obtain insight into the possible neural function of leukotriene 1341 we examined the immunohistochemical distribution of leukotriene A(4) hydrolase, an enzyme catalyzing the final and committed step in leukotriene B-4 biosynthesis, in the mouse nervous system. Immunoreactivity for leukotriene A4 hydrolase showed widespread distribution with preference to the sensory-associated structures; i.e. neurons in the olfactory epithelium and vomeronasal organ, olfactory glomeruli, possibly amacrine cells, neurons in the ganglion cell layer and three bands in the inner plexiform layer of the retina, axons in the optic nerve and tract up to the superior colliculus, inner and outer hair cells and the spiral ganglion cells in the cochlea, vestibulocochlear nerve bundle, spinal trigeminal tract, and lamina 11 of the spinal cord. Double immunofluorescence staining demonstrated that most of the leukotriene A(4)-hydrolase-immunopositive neurons coexpressed calretinin, a calcium-binding protein in neurons. The ubiquitous distribution of leukotriene A4 hydrolase was in sharp contrast with the distribution of leukotriene C-4 synthase [Shimada A, Satoh M, Chiba Y, Saitoh Y, Kawamura N, Keino H, Hosokawa M, Shimizu T (2005) Highly selective localization of leukotriene C4 synthase in hypothalamic and extrahypothalamic vasopressin systems of mouse brain. Neuroscience 131:683-689] which was confined to the hypothalamic and extrahypothalamic vasopressinergic neurons. These results suggest that leukotriene 134 may exert some neuromodulatory function mainly in the sensory nervous system, in concert with calretinin. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.