Identification, expression analysis, and the regulating function on C/EBPs of KLF10 in Dalian purple sea urchin, Strongylocentrotus nudus
Identification, expression analysis, and the regulating function on C/EBPs of KLF10 in Dalian purple sea urchin, Strongylocentrotus nudus
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大连紫海胆Strongylocentrotus nudus中KLF10的鉴定、表达分析及其对C/EBPs的调控作用
DOI：
10.1139/gen-2017-0033
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发表时间：
2017
期刊：
影响因子：
3.1
通讯作者：
Liu Xiaolin
中科院分区：
文献类型：
--
作者：
Wu Kaikai;Jia Zhiying;Wang Qi'ai;Wei Zhenlin;Zhou Zunchun;Liu Xiaolin
越来越多的证据表明，Krüppel 样因子 (KLF) 通过调节 CCAAT/增强子结合蛋白 (C/EBP) 在脂肪生物学中发挥重要作用。然而，KLFs 和 C/EBPs 尚未从裸甲鱼中鉴定出来，它们在该物种中的作用尚不清楚。在本研究中，S.的全长cDNA。 nudusKLF10 (SnKLF10) 和 S 的三个 cDNA 片段。获得 nudusC/EBP（SnC/EBP）。对性腺发育过程中组织分布和表达模式的检查表明，SnKLF10 和 SnC/EBP 在性腺脂肪生成中发挥重要作用。 SnC/EBP 中 KLF 转录因子结合位点 (TFBS) 的存在以及过表达测定的结果表明，SnKLF10 负向调节 SnC/EBP 的转录。此外，还确定了SnC/EBP的核心启动子区域，并鉴定了多个转录因子(TF)的TFBS，它们是SnC/EBP转录的潜在调节因子。综上所述，这些结果表明 SnC/EBP 基因是 SnKLF10 的潜在靶标，并且 SnKLF10 通过抑制 SnC/EBP 的转录在脂肪生成中发挥作用。这些发现为进一步研究无脊椎动物中的KLF10提供了信息，并为C/EBP转录调控机制提供了新的见解。
Accumulating evidence indicates that Krüppel-like factors (KLFs) play important roles in fat biology via the regulation of CCAAT/enhancer binding proteins (C/EBPs). However, KLFs and C/EBPs have not been identified fromStrongylocentrotus nudus, and their roles in this species are not clear. In this study, the full-length cDNA ofS. nudusKLF10 (SnKLF10) and three cDNA fragments ofS. nudusC/EBPs (SnC/EBPs) were obtained. Examination of tissue distribution and expression patterns during gonadal development implied that SnKLF10 and SnC/EBPs play important roles in gonadal lipogenesis. The presence of transcription factor-binding sites (TFBSs) for KLFs in SnC/EBPs, and the results of an over-expression assay, revealed that SnKLF10 negatively regulates the transcription of SnC/EBPs. In addition, the core promoter regions of SnC/EBPs were determined, and multiple TFBSs for transcription factor (TFs) were identified, which are potential regulators of SnC/EBP transcription. Taken together, these results suggest that SnC/EBP genes are potential targets of SnKLF10, and that SnKLF10 plays a role in lipogenesis by repressing the transcription of SnC/EBPs. These findings provide information for further studies of KLF10 in invertebrates and provide new insight into the regulatory mechanisms of C/EBP transcription.