On the promoter complex formation rate of E. coli RNA polymerases with T7 phage DNA.
On the promoter complex formation rate of E. coli RNA polymerases with T7 phage DNA.
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关于大肠杆菌 RNA 聚合酶与 T7 噬菌体 DNA 的启动子复合物形成率。
DOI：
10.1093/nar/8.6.1391
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发表时间：
1980
影响因子：
14.9
通讯作者：
M. F. Shemyakin
中科院分区：
文献类型：
--
作者：
B. N. Belintsev;S. Zavriev;M. F. Shemyakin
使用膜过滤测定法研究了离子强度对大肠杆菌 RNA 聚合酶和 T7 噬菌体 DNA 之间启动子复合物形成动力学的影响。测定酶-启动子关联速率常数。当离子强度从 0 变化到 0.15 M NaCl 时，它从 10(9) 变化到 3 x 10(7) M-1 sec-1。基于对实验数据的理论分析，讨论了假定酶沿着DNA滑动的启动子位点选择模型。
Influence of ionic strength on the kinetics of the promoter complex formation between E. coli RNA polymerase and T7 phage DNA was investigated using a membrane filter assay. The enzyme-promoter association rate constant was determined. It varies from 10(9) to 3 x 10(7) M-1 sec-1 when the ionic strength is changed from zero to 0.15 M NaCl. Basing on the theoretical analysis of experimental data obtained the model for the promoter site selection assuming the enzyme sliding along the DNA is discussed.