Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe.
Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe.
复制标题
DOI：
10.1038/nmeth.3656
复制
发表时间：
2016-01
期刊：
影响因子：
48
通讯作者：
Wang LV
中科院分区：
文献类型：
--
作者：
Yao J;Kaberniuk AA;Li L;Shcherbakova DM;Zhang R;Wang L;Li G;Verkhusha VV;Wang LV
遗传编码探针的光声层析成像（PAT）允许在深度处以高空间分辨率成像靶向生物过程。在这里，我们首次将多尺度光声成像与可逆可切换的非荧光细菌光敏色素BphP1相结合。与血红素衍生的胆绿素发色团，BphP1具有最红移的吸收报告的基因编码的探针，是可逆的光转换之间的红色和近红外光吸收状态。我们将单波长PAT与高效的BphP1光开关相结合，实现了差分成像，大大去除了背景信号，增强了检测灵敏度，增加了穿透深度，并提高了空间分辨率。在这样做的过程中，我们使用光声计算机断层扫描在接近10 mm的深度以约100 µm的分辨率监测肿瘤生长和转移，并使用光声显微镜以约140 nm的亚光学衍射分辨率对单个癌细胞进行成像。该技术有望用于不同长度尺度的生物医学研究。
Photoacoustic tomography (PAT) of genetically encoded probes allows imaging of targeted biological processes with high spatial resolution at depths. Here, we combined multi-scale photoacoustic imaging with, for the first time, a reversibly switchable non-fluorescent bacterial phytochrome BphP1. With a heme-derived biliverdin chromophore, BphP1 has the most red-shifted absorption among reported genetically encoded probes, and is reversibly photoconvertible between its red and near-infrared light absorption states. We combined single-wavelength PAT with efficient BphP1 photoswitching, enabling differential imaging that substantially removed background signals, enhanced detection sensitivity, increased penetration depth, and improved spatial resolution. In doing so, we monitored tumor growth and metastasis with a ~100 µm resolution at depths approaching 10 mm using photoacoustic computed tomography, and imaged individual cancer cells with a sub-optical-diffraction resolution of ~140 nm using photoacoustic microscopy. This technology is promising for biomedical studies at different length scales.