Mannose alleviates yellowing process of broccoli florets by regulating chlorophyll catabolism and delaying programmed cell death
Mannose alleviates yellowing process of broccoli florets by regulating chlorophyll catabolism and delaying programmed cell death
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甘露糖通过调节叶绿素分解代谢和延缓程序性细胞死亡来减轻西兰花小花的变黄过程
DOI：
10.1016/j.scienta.2022.110888
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发表时间：
2022-01-12
影响因子：
4.3
通讯作者：
Xu, Feng
中科院分区：
文献类型：
--
作者：
Guo, Fan;Han, Airu;Xu, Feng
研究了甘露糖处理对青花菜小花在20 ℃贮藏期间叶绿素催化和细胞程序性死亡（PCD）的影响。结果表明，甘露糖处理可通过调控叶绿素催化剂基因BoPPH、BoPAO、BoRCCR、BoSGR 1和BoSGR 2有效抑制青花菜叶绿素降解。同时，甘露糖处理有效地抑制了青花菜小花中依赖于拟南芥的PCD途径的发生。甘露糖处理的青花菜小花中活性氧含量减少，延缓了线粒体膜电位的升高，抑制了线粒体通透性转换孔的展开，这与抑制线粒体中细胞色素c的释放，维持高水平的细胞色素c/a、Ca 2+含量比值、细胞色素c氧化酶和交替氧化酶活性有关。这些结果表明，甘露糖处理通过抑制叶绿素的降解和PCD的出现来抑制青花菜小花的衰老。
The effect of mannose treatment on chlorophyll catabolism and programmed cell death (PCD) in broccoli florets during storage at 20 degrees C has been investigated. The results showed that mannose treatment efficiently restrained chlorophyll degradation in broccoli florets via regulating chlorophyll catabolism genes (BoPPH, BoPAO, BoRCCR, BoSGR1 and BoSGR2). Meanwhile, mannose treatment efficiently suppressed the occurrence of PCD through the mitochondria-dependent pathway in broccoli florets. Less reactive oxygen species content observed in mannose-treated broccoli florets delayed the increase of mitochondrial membrane potential and inhibited unfolding of mitochondria permeability transition pore, which was associated with the inhibition of cytochrome c liberation in mitochondria and the maintenance of high levels of cytochrome c/a and the ratio of Ca2+ content, the activities of cytochrome c oxidase and alternative oxidase. These results suggested that mannose treatment inhibited senescence of broccoli florets through suppressing the chlorophyll degradation and the appearance of PCD.