Survivin-31G/C promoter polymorphism and sporadic colorectal cancer
Survivin-31G/C promoter polymorphism and sporadic colorectal cancer
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DOI：
10.1007/s00384-008-0601-2
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发表时间：
2009-02-01
影响因子：
2.8
通讯作者：
Nikiteas, Nikolaos
中科院分区：
文献类型：
--
作者：
Gazouli, Maria;Tzanakis, Nikolaos;Nikiteas, Nikolaos
Survivin是一种凋亡抑制因子，在细胞周期调控中起重要作用，可能参与肿瘤的发生和发展。Survivin基因启动子区-31 G/C多态性与大肠癌的发生、发展及预后有关，本研究旨在探讨Survivin基因启动子区-31 G/C多态性与大肠癌发生、发展及预后的关系，采用聚合酶链反应（PCR）技术检测Survivin基因启动子区-31G/C多态性。应用限制性片段长度多态性方法对312例散发性结直肠癌患者和362例健康人的活检组织进行分析。采用定量逆转录聚合酶链反应（RT-PCR）检测结直肠癌组织中Survivin mRNA的表达，结直肠癌患者中-31GG、-31GC和-31CC基因型频率分别为21.79%、41.99%和36.22%，健康对照组分别为33.98%、45.03%和20.99%。结直肠癌患者中生存素-31C等位基因和CC基因型的频率明显高于健康受试者（p < 0.0001）。与-31GG和-31GC基因型的病例相比，-31CC基因型的纯合子表达的Survivin mRNA水平高1.6倍，Survivin启动子的-31CC基因型与CRC相关，并且可能是CRC的危险因素。
Survivin is an apoptotic inhibitor, plays an important role in cell cycle regulation, and may be involved in the development and progression of cancer. A common polymorphism at the survivin gene promoter (-31 G/C) has been shown to influence survivin expression and the risk for cancer.The aim of the present study was to investigate whether this polymorphism could be involved in the sporadic colorectal cancer (CRC) development, prognosis, and survival.The -31G/C polymorphism of survivin promoter was analyzed by polymerase chain reaction (PCR) restriction fragment length polymorphism method in biopsies from 312 patients with sporadic CRC and 362 healthy individuals. Survivin messenger RNA (mRNA) expression in CRC tissues was detected by quantitative reverse transcriptase PCR.The genotype frequencies for -31GG, -31GC, and -31CC were 21.79%, 41.99%, and 36.22% in CRC patients and 33.98%, 45.03%, and 20.99% in healthy subjects, respectively. The frequencies of the survivin -31C allele and CC genotype were significantly higher in CRC patients than in healthy subjects (p < 0.0001). Homozygotes for the -31CC survivin genotype, expressed 1.6-fold higher mRNA levels of survivin compared to cases with the -31GG and -31GC genotypes.The -31CC genotype of survivin promoter is associated with CRC and may be a risk factor for CRC.