Strategies in Ebola virus disease (EVD) diagnostics at the point of care.
Strategies in Ebola virus disease (EVD) diagnostics at the point of care.
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DOI：
10.1080/1040841x.2017.1313814
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发表时间：
2017-11
影响因子：
6.5
通讯作者：
Asghar W
中科院分区：
文献类型：
--
作者：
Coarsey CT;Esiobu N;Narayanan R;Pavlovic M;Shafiee H;Asghar W
埃博拉病毒病（EVD）是一种毁灭性的高传染性疾病，死亡率很高。该疾病是中非和西非地区的地方病，那里的实验室基础设施和训练有素的工作人员有限。最近2014年西非爆发的EVD疫情在病例数量和死亡人数方面都是前所未有的，并且已经证明这种区域性疫情可能成为对全球公共卫生的潜在威胁，因为它成为随后在西班牙和美国发生的传播事件的来源。迫切需要快速和负担得起的检测埃博拉病毒的方法，这对控制EVD的传播和防止毁灭性的死亡至关重要。目前的诊断技术包括分子诊断和其他血清学和抗原检测测定;这可能是耗时的，基于实验室的，通常需要训练有素的人员和专门的设备。在这篇综述中，我们讨论了目前使用的各种埃博拉病毒检测技术，并强调了与开发和采用新型即时诊断工具有关的潜在未来方向。最后，一个案件是需要开发新的微流控技术和多功能的快速检测平台，早期检测EVD。
Ebola virus disease (EVD) is a devastating, highly infectious illness with a high mortality rate. The disease is endemic to regions of Central and West Africa, where there is limited laboratory infrastructure and trained staff. The recent 2014 West African EVD outbreak has been unprecedented in case numbers and fatalities, and has proven that such regional outbreaks can become a potential threat to global public health, as it became the source for the subsequent transmission events in Spain and the USA. The urgent need for rapid and affordable means of detecting Ebola is crucial to control the spread of EVD and prevent devastating fatalities. Current diagnostic techniques include molecular diagnostics and other serological and antigen detection assays; which can be time-consuming, laboratory-based, often require trained personnel and specialized equipment. In this review, we discuss the various Ebola detection techniques currently in use, and highlight the potential future directions pertinent to the development and adoption of novel point-of-care diagnostic tools. Finally, a case is made for the need to develop novel microfluidic technologies and versatile rapid detection platforms for early detection of EVD.